684
GC CHROMATOGRAMS |
PHARMACEUTICAL
Pest icides in Dietar y Supplements
dSPE vs. cSPE Sample Cleanup of Dandelion Root Powder (Dietary Supplement)
Rxi®-5Sil MS
GC_FF1171
Column
Rxi
®
-5Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
Sample
Dandelion Root powder spiked with 46 pesticides
QuEChERS Internal Standard Mix for GC/MS Analysis (cat.# 33267)
anthracene for quality control (cat.# 33264)
Conc.:
80 pg/µL
Injection
Inj. Vol.:
1 µL splitless (hold 1.5 min.)
Liner:
5mm Splitless with wool (cat.# 22975-200.1)
Inj. Temp.:
250 °C
Oven
Oven Temp:
90 °C (hold 1.5 min.) to 340 °C at 8 °C/min.
Carrier Gas
He, constant flow
Flow Rate:
1.5 mL/min.
Detector
MS
Mode:
Analyzer Type:
TOF
Source Temp.:
225 °C
Electron Energy:
70 eV
Ionization Mode:
EI
Acquisition Range: 45-550 amu
Acquisition Rate:
5 spectra/sec.
Instrument
LECO Pegasus 4D GCxGC-TOFMS
Notes
Sample Preparation:
Wetting: 1 g of Dandelion Root powder combined with 9 mL of water, shaken well, fortified with pesticides and QuEChERS Internal Standard
Mix for GC/MS Analysis (cat.# 33267), soak for 2 hours.
Extraction: 10 mL acetonitrile added then addition of Q-sep™ Q110 (cat.# 26235), centrifuge with Q-sep™ 3000 Centrifuge (cat.# 26230).
dSPE: cleanup procedure according to EN 15662 method, add control standard anthracene (cat.# 33264) to 1 mL extract, add this to Q-sep™
Q110 dSPE tube (cat.# 26235), shake, centrifuge.
cSPE: add control standard anthracene (cat.# 33264) to 1 mL extract, add magnesium sulfate to cartridge, use 6mL Combo SPE Cartridge
containing 500mg CarboPrep
®
90/500mg PSA (cat.# 26194), concentrate via evaporation.
NEW!
free
literature
Developing NewMethods for Pesticides in Dietary Supplements
Advantages of the QuEChERS Approach
QuEChERS is a simple, effective approach to sample prep that can be applied to the analysis
of pesticides in dietary supplements. Here we demonstrate a QuEChERS, cSPE, GC-TOFMS
procedure that results in good recoveries for a wide range of pesticides in dandelion root.
Download your copy from
Applications Note
lit. cat.# PHAN1242
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
