674
GC CHROMATOGRAMS |
CLINICAL, FORENSIC & TOXICOLOGY
Opiates, Cannabinoids
Derivatized Cannabinoids (5 μg/mL)
Rxi®-5Sil MS
Column
Rxi
®
-5Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
Sample
Diluent:
Ethyl acetate
Conc.:
5 µg/mL TMS derivatives
Injection
Inj. Vol.:
1 µL splitless (hold 1 min.)
Liner:
3.5mm Gooseneck Splitless w/Wool (cat.# 22286-200.1)
Inj. Temp.:
250 °C
Purge Flow:
21.4 mL/min.
Oven
Oven Temp:
150 °C to 330 °C at 15 °C/min. (hold 3 min.)
Carrier Gas
He, constant linear velocity
Linear Velocity: 40 cm/sec., 13.8 psi, 95.1kPa @ 150 °C
Detector
MS
Mode:
Scan
Transfer Line
Temp.:
280 °C
Analyzer Type: Quadrupole
Source Temp.:
200 °C
Electron Energy: 70 eV
Solvent Delay
Time:
4 min.
Tune Type:
PFTBA
Ionization Mode: EI
Scan Range:
200-500 amu
Scan Rate:
5 scans/sec.
Instrument
Shimadzu 2010 GC & QP2010+ MS
GC_CF1145
Excellent peak shape
with short run time.
NEW!
Peaks
1. Cannabidiol
2. delta 9 THC
3. Cannabinol
4. THC-COOH; THCA
GC_CF1150
Extracted Opiates from Blood
Rxi®-5Sil MS
Peaks
1. Hydrocodone
2. Codeine
3. Oxycodone
4. Hydromorphone
5. 6-Monoacetylmorphine
6. Morphine
* Matrix
Column
Rxi
®
-5Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
Sample
Diluent:
Ethyl acetate
Conc.:
100 ng/mL propionic anhydride derivatives
Injection
Inj. Vol.:
1 µL splitless (hold 1 min.)
Liner:
Gooseneck Splitless (4mm) w/Wool (cat.# 22405)
Inj. Temp.:
250 °C
Purge Flow:
100 mL/min.
Oven
Oven Temp:
65 °C (hold 1 min.) to 315 °C at 15 °C/min.
Carrier Gas
He, constant flow
Flow Rate:
1 mL/min.
Linear Velocity:
35 cm/sec. @ 65 °C
Detector
MS
Mode:
SIM
Transfer Line
Temp.:
250 °C
Analyzer Type:
Quadrupole
Solvent Delay
Time:
7 min.
Tune Type:
PFTBA
Ionization Mode: EI
Notes
Opiates were spiked into a blood sample and extracted
by SPE, then derivatized with propionic anhydride.
Acknowledgement Data courtesy of Miami Dade County
Medical Examiner Department
NEW!
Rxi® Technology!
Exceptionally inert,
ultra low- bleed
capillary columns.
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E-mail :
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Mar 2011
