673
GC CHROMATOGRAMS |
CLINICAL, FORENSIC & TOXICOLOGY
Cocaine, Opiates
Cocaine & Metabolites (TMS Derivatives)
Rtx®-5
min. 2
4
6
8
10
12
14
16
GC_PH00254
5
3
4
Column:
Rtx
®
-5, 30m, 0.25mm ID, 0.25µm (cat.# 10223)
Sample:
1.0µL split injection of cocaine and cocaine metabolites
Oven temp.:
150°C to 320°C @ 10°C/min.
Inj./det. temp.:
250°C/300°C
Carrier gas:
helium
Linear velocity:
30cm/sec. set @ 50°C
FID sensitivity:
2.56 x 10
-10
AFS
Split ratio:
30:1
1. ecgonine methyl ester (TMSI)
2. ecgonine (TMSI)
3. cocaine
4. coca ethylene
5. benzoylecgonine (TMSI)
2
1
Opiates (TMS Derivatives)
Rtx®-5
1 2
3
4
5
6
7,8
9 10
11
min.
4
5
6
7
8
9
10
11
12
GC_PH00233
1. meperidine
2. alphaprodine
3. methadone
4. levorphanol (TMS)
5. codeine (TMS)
6. hydrocodone
7. morphine (TMS)
8. hydromorphone (TMS)
9. oxycodone (TMS)
10. oxymorphone (TMS)
11. nalorphine (TMS)
Column:
Rtx
®
-5, 30m, 0.25mm ID, 0.25µm (cat.# 10223)
Sample:
2.0µL split injection of opiates
Conc:
2,000ng/µL
Oven temp.:
200°C to 325°C @ 7°C/min.
Inj./det. temp.:
250°C/300°C
Det. type:
MS
Ionization:
EI
Carrier gas:
helium
Mode:
full scan
Linear velocity:
30cm/sec. set @ 200°C
Split ratio:
50:1
Cocaine and Metabolites (TMS Derivatives)
Rxi®-5Sil MS (100 ng/mL)
Column
Rxi
®
-5Sil MS, 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
Sample
Diluent:
Butyl chloride
Conc.:
100 ng/mL
Injection
Inj. Vol.:
1 µL splitless (hold 1 min.)
Liner:
Single Gooseneck w/Wool (cat.# 22286-200.1)
Inj. Temp.:
250 °C
Purge Flow:
20 mL/min.
Oven
Oven Temp:
100 °C to 200 °C at 30 °C/min. to 300 °C at 15 °C/min.
Carrier Gas
He, constant linear velocity
Linear Velocity: 40 cm/sec., 12.5 psi, 86.2kPa @ 100 °C
Detector
MS
Mode:
SIM
Transfer Line
Temp.:
310 °C
Source Temp.:
250 °C
Solvent Delay
Time:
4 min.
Tune Type:
PFTBA
Ionization Mode: EI
Instrument
Shimadzu 2010 GC & QP2010+ MS
Notes
Samples were prepared as follows:
Standards brought to dryness under nitrogen, then 50 µL
BSTFA + 1%TMCS (cat.# 35606) added. 50 µL pyridine
was then added, and samples were incubated at 70°C for
30 min. After incubation, samples were diluted with butyl
chloride.
* Used as derivitazation check
GC_CF1149
NEW!
Peaks
m/z 1 m/z 2
m/z 3
1. Ecgonine methyl ester
82
96
256
2. Ecgonine
82
96
356
3. Cocaine
82
182
303
4. Cocaethylene
82
196
317
5. Benzoylecgonine
82
96
346
6. Cannabinol*
409
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Mar 2011
