667
GC CHROMATOGRAMS |
CLINICAL, FORENSIC & TOXICOLOGY
Blood Alcohols
Blood Alcohol
5% Carbowax® 20M on CarboBlack B
(packed; headspace analysis)
Column:
5% Carbowax
®
20M on 80/120 CarboBlack B,
6 ft.,
1
/
8
", 2.0mm ID SilcoSmooth
®
tubing
(cat.# 80105)
Inj.:
500µL, headspace
Conc.:
0.05gm/dL
Oven temp.:
90°C
Inj./det. temp.:
200°C
Carrier gas:
helium
Flow rate:
18mL/min.
1. acetaldehyde
2. methanol
3. acetone
4. ethanol
5. isopropanol
6.
tert
-butanol
GC_PH00478
Blood Alcohol
Rtx®-BAC1 & Rtx®-BAC2 (0.32 mm ID Columns)
(headspace analysis)
Dual-column analysis using a two-hole ferrule.
Sample:
1.0mL headspace sample of a blood alcohol mix
on a PerkinElmer HS 40 headspace autosampler
Oven temp.:
40°C
Inj. temp.:
200°C
Carrier gas:
helium
Sample equilibration:
70°C, 15 min.
Vial pressure:
30psi
Vial pressurization time: 0.15 min.
Vial sampling time:
0.01 min.
Transfer line:
0.32mm ID Hydroguard
®
fused silica tubing
Transfer line temp.:
200°C
Injection port sleeve:
2mm ID
Split flow:
20mL/min.
Conc.
w/v
1. methanol
0.1%
2. acetaldehyde
0.2%
3. ethanol
0.2%
4. isopropanol
0.1%
5. acetone
0.01%
6.
n
-propanol
0.1%
Rtx®-BAC1, 30m, 0.32mm ID, 1.8μm (cat.# 18003)
Rtx®-BAC2, 30m, 0.32mm ID, 1.2μm (cat.# 18002)
min.
1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0
6
5
4
3
2
1
6
5
4
3
2
1
GC_PH00241
min.
1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
