685
GC CHROMATOGRAMS |
PHARMACEUTICAL
Pest icides in Dietar y Supplements
Dietary Supplement (Finished Product) QuEChERS Extract Cleaned with Cartridge SPE
(GCxGC-TOFMS Contour Plot)
GC_FF1191
Column
Rxi
®
-5Sil MS 30 m, 0.25 mm ID, 0.25 µm (cat.# 13623)
Rtx
®
-200 1.5 m, 0.18 mm ID, 0.20 µm (cat.# 45001)
Sample
Diluent:
Toluene
Injection
Inj. Vol.:
1 µL splitless (hold 1 min.)
Liner:
Gooseneck Splitless (4mm) w/Wool (cat.# 22405)
Inj. Temp.:
250 °C
Purge Flow:
40 mL/min.
Oven
Oven Temp:
Rxi
®
-5Sil MS: 80 °C (hold 1 min.) to 310 °C at 4 °C/min. (hold 1.5 min.)
Rtx
®
-200: 90 °C (hold 1 min.) to 320 °C at 4 °C/min. (hold 1.5 min.)
Carrier Gas
He, constant flow
Flow Rate:
1.8 mL/min.
Modulation
Modulator
Temp. Offset:
25 °C
Second Dimension
Separation Time: 4 sec.
Hot Pulse Time:
1.2 sec.
Cool Time
between Stages: 0.8 sec.
Detector
TOFMS
Transfer
Line Temp.:
290 °C
Analyzer Type:
TOF
Source Temp.:
225 °C
Electron Energy: 70 eV
Mass Defect:
-20 mu/100 u
Solvent Delay
Time:
4 min.
Ionization Mode: EI
Acquisition Range: 45 to 550 amu
Spectral
Acquisition Rate: 100 spectra/sec
Instrument
LECO Pegasus 4D GCxGC-TOFMS
Notes
See application note PHAN1251 for extraction and cleanup details. A 1.5 m length of the Rtx
®
-200 column was trimmed from a
10 m column. Columns were connected with a Universal Press-Tight
®
Connector (cat.# 20429).
GCxGC with orthogonal Rxi®-5Sil MS and Rtx®-200 columns is a powerful
way to handle complex samples like dietary supplement extracts.
NEW!
Restek is your
complete source
for analyzing
pesticides in
dietary supplements.
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
