625
GC CHROMATOGRAMS |
FOODS, FLAVORS & FRAGRANCES
Cholesterols, Essent ial Oi ls
Underivatized Cholesterol
Rxi®-5ms
GC_FF00881
GC_FF00882
B. Isothermal conditions will maximize sample throughput.
Oven temp.:
300°C (hold 10 min.)
A. Temperature program conditions.
Oven temp.:
200°C (hold 1 min.) to 330°C @
20°C/min. (hold 7.5 min.)
Column:
Rxi
®
-5ms, 15m, 0.25mm ID, 0.25µm (cat.# 13420)
Sample:
1,000µg/mL cholesterol in DMF, 1000µg/mL 5-
α
-cholestane in hexane;
25ng cholesterol, 150ng 5-
α
-cholestane on column
Inj.:
1.0µL, split (20:1), single gooseneck inlet liner w/wool (cat.# 22405)
Inj. temp.:
250°C
Carrier gas:
helium, constant pressure (9.7psi @ 200°C)
Linear velocity: 24cm/sec.
Oven temp.:
see above
Det.:
FID @ 340°C
1. 5-
α
-cholestane (IS)
2. cholesterol
Derivatized Cholesterol
Rxi®-5ms
B. Isothermal conditions will maximize
sample throughput.
Oven temp.:
300°C (hold 10 min.)
A. Temperature program conditions.
Oven temp.:
200°C (hold 1 min.) to 330°C @
20°C/min. (hold 7.5 min.)
Column:
Rxi
®
-5ms, 15m, 0.25mm ID, 0.25µm (cat.# 13420)
Sample:
1,000µg/mL cholesterol in hexane, 1000µg/mL 5-
α
-cholestane in hexane;
50ng derivatized cholesterol, 150ng 5-
α
-cholestane on column
Inj.:
1.0µL, split (20:1), single gooseneck inlet liner w/wool (cat.# 22405)
Inj. temp.:
250°C
Carrier gas:
helium, constant pressure (9.7psi @ 200°C)
Linear velocity: 24cm/sec.
Oven temp.:
see above
Det.:
FID @ 340°C
1. 5-
α
-cholestane (IS)
2. cholesterol
GC_FF00883
GC_FF00884
One column, many
cholesterol methods.
2 3 5
19
Lemon Oil
Rtx®-5
GC_FF00138
min.
2
4
6
8
10
12
14
16
18
20
22
24
26
28
1
4
6
9
7,8
11
10
12
13
14
15
16
17
18
20
21 22
23
24
25
26
27,28
29
30
31
32
33
34
35
36
38
37
1. heptanol
2.
α
-thujene
3.
α
-pinene
4. camphene
5. sabinene
6.
β
-pinene
7. 6-methyl-5-hepten-2-one
8. myrcene
9. octanal
10.
α
-phellandrene
11. 3-carene
12.
α
-terpinene
13.
p
-cymene
14. limonene
15.
γ
-terpinene
16. octanol
17. terpinolene
18. linalool
19. nonanal
20. citronellal
21. terpinene-4-ol
22.
α
-terpineol
23. decanol
24. octyl acetate
25. nerol
26. neral
27. carvone
28. geraniol
29. geranial
30. nonyl acetate
31. citronellyl acetate
32. neryl acetate
33. geranyl acetate
34. dodecanal
35.
β
-caryophyllene
36.
trans
-
α
-bergamotene
37.
α
-humulene
38.
β
-bisabolene
Column:
Rtx
®
-5, 30m, 0.32mm ID, 0.25µm (cat.# 10224)
Sample:
Wet needle split injection of a neat lemon oil
Oven temp.:
75°C (hold 8 min.) to 250°C @ 4°C/min.
Inj./det. temp.:
250°C
Carrier gas:
hydrogen
Linear velocity:
40cm/sec. (flow rate: 3.2cc/min.)
FID sensitivity:
2 x 10
-11
AFS
Split ratio:
100:1
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
