618
GC CHROMATOGRAMS |
FOODS, FLAVORS & FRAGRANCES
FAMEs
FAMEs (NLEA Mix)
Rt®-2560
Column:
Rt
®
-2560, 100m, 0.25mm ID, 0.20µm (cat.# 13199)
Sample:
NLEA FAME Mix (cat.# 35078), 30mg/mL
total FAMEs in methylene chloride
Inj.:
1.0µL split (split ratio 100:1),
4mm inlet liner (cat.# 20814)
Inj. temp.:
225°C
Carrier gas:
hydrogen, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
100°C (4 min. hold) to 240°C @ 3°C/min. (10 min. hold)
Det.:
FID @ 250°C
1. C4:0 methyl butyrate
2. C6:0 methyl hexanoate
3. C8:0 methyl octanoate
4. C10:0 methyl decanoate
5. C11:0 methyl undecanoate
6. C12:0 methyl laurate
7. C13:0 methyl tridecanoate
8. C14:0 methyl myristate
9. C14:1 methyl myristoleate (
cis
-9)
10. C15:0 methyl pentadecanoate
11. C16:0 methyl palmitate
12. C16:1 methyl palmitoleate (
cis
-9)
13. C17:0 methyl heptadecanoate
14. C18:0 methyl stearate
15. C18:1 methyl elaidate (
trans
-9)
16. C18:1 methyl oleate (
cis
-9)
17. C18:2 methyl linoelaidate (
trans
-9,12)
18. C18:2 methyl linoleate (
cis
-9,12)
19. C20:0 methyl arachidate
20. C20:1 methyl eicosenoate (
cis
-11)
21. C18:3 methyl linolenate (
cis
-9,12,15)
22. C22:0 methyl behenate
23. C22:1 methyl erucate (
cis
-13)
24. C23:0 methyl tricosanoate
25. C24:0 methyl lignocerate
26. C20:5 methyl eicosapentaenoate (
cis
-5,8,11,14,17)
27. C24:1 methyl nervonate (
cis
-15)
28. C22:6 methyl docosahexaenoate (
cis
-4,7,10,13,16,19)
GC_FF00651
NLEA FAME Mix
cat.# 35078
An Rt®-2560 column provides
the resolution of
cis
&
trans
FAMEs needed for AOAC
Method 996.06.
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Mar 2011
