619
GC CHROMATOGRAMS |
FOODS, FLAVORS & FRAGRANCES
FAMEs
GC_FF00652
FAMEs (
cis
/
trans
isomers)
Rt®-2560
Column:
Rt
®
-2560, 100m, 0.25mm ID, 0.2µm (cat.# 13199)
Sample:
cis
/
trans
FAME Mix (cat.# 35079), 10mg/mL total FAMEs in methylene chloride
Inj.:
1.0µL split (split ratio 20:1), 4mm inlet liner (cat.# 20814)
Inj. temp.:
225°C
Carrier gas:
hydrogen, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
100°C (4 min. hold) to 240°C @ 3°C/min. (10 min. hold)
Det.:
FID @ 250°C
Compound
% in Mix
1. C18:0 methyl stearate
20.0
2. C18:1 methyl petroselaidate (
trans
-6)
8.0
3. C18:1 methyl elaidate (
trans
-9)
10.0
4. C18:1 methyl transvaccenate (
trans
-11)
12.0
5. C18:1 methyl petroselinate (
cis
-6)
8.0
6. C18:1 methyl oleate (
cis
-9)
10.0
7. C18:1 methyl vaccenate (
cis
-11)
12.0
8. C18:2 methyl linoleate (
cis
-9,12)
20.0
cis
/
trans
FAME Mix
cat.# 35079
Excellent resolution of
cis
&
trans
isomers!
5,6
FAMEs (
cis
/
trans
isomers)
Rtx®-Wax
Column:
Rtx
®
-Wax, 60m, 0.25mm ID, 0.25µm (cat.#12426)
On-column conc.:
40–75ng
Oven temp.:
165°C to 250°C @ 2°C/min.
Inj./det. temp.:
220°C/250°C
Carrier gas:
helium
Linear velocity:
20cm/sec. set @ 165°C
Split ratio:
50:1
1
3
2
4
7
8
9
10
12
11
13
14
1. C14:0
2. C14:1n5
cis
3. C14:1n5
trans
4. C16:0
5. C16:1n7
cis
6. C16:1n7
trans
7. C18:0
8. C18:1
cis
isomers
(n12, n9, n7)
9. C18:1
trans
isomers
(n12, n9, n7)
10. C18:2n6
cis
11. C18:2n6
trans
12. C20:0
13. C20:1n9
cis
14. C20:1n9
trans
min. 12
14
16
18
20
22
24
26
28
30
32
34
36
GC_FF00121
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
