621
GC CHROMATOGRAMS |
FOODS, FLAVORS & FRAGRANCES
FAMEs
FAMEs (Cocoa Butter)
Stabilwax
®
Column:
Stabilwax
®
, 30m, 0.25mm ID, 0.25µm (cat.# 10623)
Sample:
1.0µL split injection of a FAME reference standard for
cocoa butter.
Oven temp.:
200°C to 250°C @ 8°C/min. (hold 3 min.)
Inj./det. temp.:
250°C
Carrier gas:
hydrogen
Linear velocity:
31.4cm/sec. set @ 200°C
FID sensitivity:
8 x 10
-11
AFS
Split ratio:
45:1
1. C14:0
2. C16:0
3. C16:1n7
4. C17:0
5. C18:0
6. C18:1n9
7. C18:2n6
8. C18:3n3
9. C20:0
10. C20:1n9
11. C22:0
GC_FF00120
1
3
2
6
7
5
9
10
11
4
min.
2
4
6
8
8
GC_FF00538
Column:
Rtx
®
-Wax, 30m, 0.25mm, 0.25µm (cat.# 12423)
Sample:
1µL split injection of saw palmetto standard
Conc.:
see peak list
Oven temp.:
120°C (hold 3 min.) to 220°C
at 20°C/min. (hold 12 min.)
Inj./det. temp.:
250°C/300°C
Carrier gas:
helium
Linear velocity:
1mL/min. (34 cm/sec.)
Split ratio:
100:1
FAMEs (Saw Palmetto)
Rtx®-Wax
Peak List
Conc. (mg/mL)
1. methyl caproate (C6:0)
0.4
2. methyl caprylate (C8:0)
0.4
3. methyl nonanoate (C9:0)
2.0
4. methyl caprate (C10:0)
0.4
5. methyl laurate (C12:0)
5.0
6. methyl myristate (C14:0)
2.0
7. methyl palmitate (C16:0)
2.0
8. methyl palmitoleate (C16:1)
0.4
9. methyl stearate (C18:0)
0.4
10. methyl oleate (C18:1)
5.0
11. methyl linoleate (C18:2)
1.0
12. methyl linolenate (C18:3)
0.4
5
FAMEs (PUFA, animal source)
Rt®-2330
1. C14:0
2. C16:0
3. C16:1n7
4. C18:0
5. C18:1n9
6. C18:1n7
7. C18:2n6
8. C18:3n6
9. C18:3n3
10. C20:3n6
11. C20:4n6
12. C20:5n3
13. C22:4n6
14. C22:5n3
15. C22:6n3
Column:
Rt
®
-2330, 30m, 0.32mm ID, 0.20µm
(cat.# 10724)
Sample:
0.1µL split injection of PUFA 2 mix
Oven temp.:
160°C to 250°C @ 2°C/min. (hold 10 min.)
Inj./det. temp.:
260°C
Carrier gas:
hydrogen
Linear velocity:
40cm/sec.
FID sensitivity:
8 x 10
-11
AFS
Split ratio:
20:1
min.
4
8
12
16
20
24
28
1
2
3
4
6
7
8
9
10
11
12
13
14
15
GC_FF00119
8
7
13
FAMEs (PUFA, marine source)
Rt®-2330
Column:
Rt
®
-2330, 30m, 0.25mm ID, 0.20µm
(cat.# 10723)
Sample:
0.5µL split injection of a PUFA mix
Oven temp.:
160°C to 225°C @ 2°C/min.
Inj./det. temp.:
225°C/250°C
Carrier gas:
hydrogen
Linear velocity:
45cm/sec. set @ 160°C
FID sensitivity:
8 x 10
-11
AFS
Split ratio:
35:1
1
2
3
4
6
5
9
11
10
12
14
15
16
17
18
min.
5
10
15
20
GC_FF00118
1. C14:0
2. C16:0
3. C16:1n7
4. C18:1n9
5. C18:1n7
6. C18:2n6
7. C18:3n3
8. C20:1n11
9. C20:1n9
10. C20:1n7
11. C18:4n3
12. C22:1n11
13. C22:1n9
14. C20:4n3
15. C20:5n3
16. C21:5n3
17. C22:5n3
18. C22:6n3
Website :
E-mail :
TelNo : 03 9762 2034 . . . in AUSTRALIA
Mar 2011
