Thischromatogramshowsastepgradient
beginningwithpumpAat100%andending
withpumpB at 100%. Eachof the five
20%steps isheldforfiveminutes.
Gradientprogram:
%pumpAHold Ramp
%pumpB
100.00 5.00 0.00 0.00
80.00 5.00 0.00 20.00
60.00 5.00 0.00 40.00
40.00 5.00 0.00 60.00
20.00 5.00 0.00 80.00
0.00 5.00 0.00 100.00
Description:UV254
Column:15cmC185µ
Mobilephase:methanolat2.0mL/minute
PumpA: 100%methanol
PumpB: 99.75%methanol with 0.25%
acetone
Events:
Time Event
0.00 Zero100
HPLC
Model 210D
ExpectedPerformance
StepGradient
This chromatogram shows the
210DUVdetector response toa
BTX sample (benzene, toluene,
andorthoxylene).
Results:
Component Retention Area
Benzene 2.833 851.1640
Toluene
3.316 912.9170
OrthoXylene 3.816 1207.1795
Total
2971.2605
Description:UV254
Sample:20µL100ppmBTX
Column:15cmC185µ
Mobile phase: 70% Isopropyl Alcohol,
30%waterat1.0mL/minute
Detectorcell temperature:40
o
C
Column temperature:40
o
C
Pressure:1300psi
