3. Solvent Set-up
Thepumpheadseachhavean1/8”Teflonsolventinletline. Place
theendsoftheselinesinyoursolventbottle. Thesolventrecycle
valvehastwooutletlines. The1/16”clearlineisrecycledsolvent.
Putitintothesolventbottle,orintoitsowncontainerifyouwish
tokeep itseparatefromyourfreshsolvent. Place theendof the
1/16”greenlineintoawastebottle.Asimplifiedwaytoremember:
all theclear linesgo to thesolventbottle. Indefaultmode, the
solventrecyclingvalvesendstheeffluentoutthecleartube;when
activated, itdirects theeffluentout thegreenwaste tube.
Green=
towaste
Clear=
recycled
solventout
Blue= in
fromcolumn
Solvent recyclevalve
The1/4”Tygon tubingattached to thepump
head(s)isthepistonflushline.Pistonflushing
helps keep thehighpressure seal(s) clean,
minimizingwearandtearfromcrystallizedand
abrasivebufferdeposits that leak through the
seal,andwouldotherwisedryon thepiston.
There are inlet and outlet lines for piston
flushing.Place theendsofboth intoabottle
containingatleast50%organicsolvent(usually
dilutedwithdeionizedwater).Binarygradient
pumpheadpistonflushlinesaredaisychained,
andshare thesame inandout lines.
HPLC
Model 210D
210Dpump,sideview:
Piston flush
outlet line
1/4” tygon tubing
piston flush inlet line
Piston
Piston flush IN
Piston flushOUT
Lowpressureseal
Pump inlet
Pumpoutlet
It is possible for solvent to leak past
the high pressure seal with the back
and forth thrustingof thepiston.
Highpressureseal
