16. Injecting aSample andStarting theRun
TheRheodyne injectionvalvecomeswith itsownblunt-tippedflushingneedle,
which isshipped inserted into thevalveunder theprotectiveredcap. Remove the
protective cap and tape, thenpull out theneedle. Theprovided100µLglass
injectionsyringeshouldsuitmostinjectionapplications. Forthebestresults,prepare
your sample in thesamesolventyouareusing foryourmobilephase. Fill the
syringewithsample, andeliminateanyairbubbles. Insert thesyringe into the
Rheodyneinjectionvalve,pushinggentlyuntiltheneedlehitsthestop. Depressthe
syringeplungertofillthe20µLsampleloop. Leavethesyringeinthewhileyouturn
the Rheodyne injection valve knob to the INJECT position. The run will
automaticallystart. See theRheodynedocumentation inyourSRImanualunder
HPLCInjectorsformore informationaboutoperating the injectionvalve.
HPLC
Model 210D
Thedetector exit tube that connects to the topof the
solvent recycle valve may be disconnected, and
connected toexternaldetectors, likerefractive indexor
fluoresencedetectors.Theexternaldetector’sexittubes
are thenconnectedback to thesolvent recyclevalve.
210D
detector
cell
Solvent
recycle
valve
External
detector(s)
Waste
Solvent
15. Solvent Recycle Control
RelayCcontrols thesolventrecyclevalve. WhenRelayC
isON, thesolvent isdirected towaste (greenexit tube).
TurnRelayCONbeforeanypeaksappearonscreen,and
turn itOFFat theendof the run. ClickonRelayC in the
Relays/pumpwindow to turn itONandOFF, or usean
eventtabletodoitautomatically. Thesolventrecyclevalve
may alsobeused as a singlepeak fraction collector by
turning itON/OFFatspecific timesduring therun.
Exampleevent table for the
solvent recyclevalve
