HPLC
Model 210D
14. Priming thePump
Note:
Donotopen theprime/purgevalveuntilpressure is
atzero,orveryclose.Otherwise, systempressurecould
damage thepulsedamperorcause thecolumnadsorbent
bed toshift.
Open theprime/purgevalveby turning it to the left, then
startthepumpbyclickingonRelayAinthepumpwindow.
Inserttheprimingsyringeintotheprime/purgevalve, then
slowlyandgentlypullbacktheplunger.Whenyoucanpull
abubble-lessstreamoutof theprime/purgevalve, close
thevalveby turning itback to the right.Thepressureshouldbeginbuilding tooperating range,which is
dependentupon the flow rate, solventviscosity, and thebackpressureof thecolumnselected.When the
systemstabilizesat theoperatingpressure range,youmay injectyoursample. It isnormal for thepressure
gaugeneedle tofluctuatewith thepumpstroke.
13.
Click
View
andchoose
Relay/pumpwindow
. In theRelays/pumpwindow, clickonRelayAto turn the
pumpOFF,sinceyouhaven’tprimed ityet. Theflowrateof thepump(pumpAinabinarygradientsystem)
canbeadjustedfrom0.1 to5.0mL/minute, in incrementsof0.1mL/minute,using thedialogboxat thebottom
oftheRelays/pumpwindow. Inabinarygradientsystem,thegradientprogramdetermineshowtheflowissplit
between the twopumps.
Pumpspeed
