CLINICAL/FORENSICS
21
Antidepressants; Antihistamines
HPLC
Antidepressants
Ultra IBD
0
2
4
6
8 min.
Peak List:
Conc. (µg/mL)
1. uracil
5
2. maleate
5
3. benzoic acid
50
4. nortriptyline
50
5. amitriptyline
50
6. trimipramine
50
LC_0057
1
2
3
4
5
6
0
5
10
15
20
25min.
LC_0056
1
3
2
6
4
5
Sample:
Inj.:
10µL
Solvent:
mobile phase
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
20mM KH
2
PO
4
pH 3:
acetonitrile (70:30, v/v)
or20mM K
x
H
y
PO
4
, pH 7:
acetonitrile (65:35, v/v)
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
Anithistamines
Antihistamines and decongestants are common ingredients in over-the-counter coldmedications.Many include amine func-
tional groups and are classified as basic compounds.OurRtx
®
-5Amine column is speciallydesigned for basic compounds: anti-
histamines andother compounds that tail or are adsorbedonpoorlydeactivated columns exhibit excellent peak shape andbet-
ter response on anRtx
®
-5Amine column.
GC
1. phenylpropanolamine
2. ephedrine
3. pseudoephedrine
4. pheniramine
5. diphenhydramine
6. doxylamine
7. phenyltoloxamine
8. methapyrilene
9. chlorpheniramine
10. brompheniramine
11. triprolidine
30m, 0.32mm ID, 1.0µmRtx
®
-5 Amine (cat.# 12354)
1.0µL split injection of antihistamines.
Conc.:
1000ng/µL
Oven temp.:
130°C (hold 5min.) to 305°C
@ 10°C/min. (hold 5min.)
Inj. & det. temp.:
305°C
Carrier gas:
hydrogen
Linear velocity:
43cm/sec. set@ 130°C
FID sensitivity:
6.4 x 10
-11
AFS
Split ratio:
50:1
GC_PH00247
min.
5
10
15
20
25
1
2
11
10
9
8
7
6
5
4
3
pH3
pH7
Antihistamines (Underivatized)
Rtx®-5Amine
