CLINICAL/FORENSICS
20
Antidepressants (Tricyclic)
HPLC
HPLC
Antidepressants (BasicDrugs)
Allure™Basix
0
1
2
3
4
5
6
7
8
9 min.
1
2
4
5
7
3
6
LC_0053
Peak List:
1. doxepin
2. nordoxepin
3. amitriptyline
4. imipramine
5. desimipramine
6. nortriptyline
7. protriptyline
Sample:
Inj.:
10µL
Conc.:
100µg/mL each
Solvent:
mobile phase
Column:
Allure
™
Basix
Cat.#:
9161565
Dimensions: 150 x 4.6mm
Particle size: 5µm
Pore size:
60Å
Conditions:
Mobile phase: acetonitrile:
50mM KH
2
PO
4
pH3:methanol
(60:25:15, v/v)
Flow rate:
1.2mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
1
2
0
1
2
3
4
5
6
7
8
9 min.
LC_0112
Peak List:
1. desipramine
2. trimipramine
Sample:
Inj.:
10µL
Conc.:
10µg/mL
Solvent:
water:methanol (1:1, v/v)
Column:
Allure
™
Basix
Cat.#:
9161565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
5mM ammonium
formate, pH 3:methanol
(25:75, v/v)
Flow:
1.0mL/min.
Split ratio:
1:1
Column temp.:
ambient
Det.:
PE/Sciex API 150 EX
Interface:
heated nebulizer
Ionmode:
negative
Ion spray voltage: 3000V
Temp.:
400°C
Orifice:
-40V
Ring:
-100V
Auxiliary gas:
6000cc/min.
Antidepressants (BasicDrugs)
Allure™Basix (LC/MS)
GC
TricylicAntidepressants
Antidepressants are similar to phenothiazines in
structure except that the center ring is seven
membered instead of six, and does not contain
sulfur. Short GC columns operated at elevated
temperatures produce the best separations in the
shortest time. Rtx
®
-1701 columns provide a
unique selectivity for the antidepressants, per-
forming the separation isothermally in less than
sixminutes.
Tricylic antidepressants alsoare rapidly separated
onanHPLCcolumn speciallydeveloped forbasic
compounds:Allure
™
Basix.
1. amitriptyline
2. trimipramine
3. imipramine
4. nortriptyline
5. doxepin
6. desipramine
7. maprotyline
8. clomipramine
1
2
3
4
5
6
7
8
min. 1
2
3
4
5
6
Antidepressants (BasicDrugs)
Rtx®-1701
15m, 0.25mm ID, 0.25µmRtx
®
-1701 (cat.# 12020)
1.0µL split injection of antidepressants
Conc.:
25ng/component
Oven temp.:
225°C
Inj. / det. temp.:
250°C / 260°C
Carrier gas:
helium
Linear velocity:
30cm/sec. set@ 225°C
FID sensitivity:
2.56 x 10
-10
AFS
Split ratio:
40:1
GC_PH00264
