CLINICAL/FORENSICS
28
Chiral Drugs
GC
GC
GC
GC
Fenafluramine (TFAADerivative)
Rt-
β
DEXcst™
min.
15
20
25
30
35
40
30m, 0.32mm ID, 0.25µm
Rt-
β
DEXcst
™
(cat.# 13102)
On-column injection, 25ng/enantiomer
Oven temp.:
90°C (hold 1min.) to 200°C
@ 2°C/min. (hold 3min.)
Carrier gas:
hydrogen
Linear velocity:
80cm/sec. set@ 60°C
Det.:
FID set@ 220°C
1. (+)-fenfluramine -TFAA
2. (-)-fenfluramine -TFAA
3. (+)-norfenfluramine -TFAA
4. (-)-norfenfluramine -TFAA
1
2
3
4
GC_PH00244
Barbiturates (Underivatized)
Rt-
β
DEXcst™
min.
10
20
30
40
50
30m, 0.32mm ID, 0.25µm
Rt-
β
DEXcst
™
(cat.# 13102)
On-column conc.:25ng/enantiomer
Oven temp.:
60°C (hold 1min.) to
220°C@ 3°C/min.
Carrier gas:
hydrogen
Linear velocity:
80cm/sec. set@ 60°C
Det.:
FID set@ 220°C
1. (+/-)-hexobarbital
2. (+/-)-mephobarbital
1
2
GC_PH00245
Amphetamine&Methamphetamine
(TFAADerivatives)
Rt-
β
DEXcst™
10
12
14
16
18
20
22
24
min.
GC_PH00246
1. (+)methamphetamine -TFAA
2. (-)methamphetamine -TFAA
3. (+)amphetamine -TFAA
4. (-)amphetamine -TFAA
1
2
3 4
30m, 0.32mm ID, 0.25µmRt-
β
DEXsm
™
(cat.# 13104)
On-column injection
Conc.:
125ng each enantiomer
Oven temp.:
175°C to 200°C@ 2°C/min.
Inj./det. temp.:
200°C/230°C
Carrier gas:
helium
Linear velocity:
60cm/sec.
Det.:
GC-FID
Split ratio:
13:1 using cup splitter liner (cat.# 20709)
min.
7
8
(+/-)ibuprofen
Ibuprofen (Underivatized)
Rt-
β
DEXsm™
GC_PH00242
30m, 0.32mm ID, 0.25µmRt-
β
DEXcst
™
(cat.# 13102)
On-column injection
Conc.:
approximately 15ng each enantiomer
Oven temp.:
200°C to 230°C@ 1°C/min.
Inj./det. temp.:
200°C/230°C
Carrier gas:
hydrogen
Linear velocity:
80cm/sec.
Detector type:
GC/FID
Split ratio:
13:1 using cup splitter liner (cat.# 20709)
min.
20
25
(+/-)thalidomide
Thalidomide (Underivatized)
Rt-
β
DEXcst™
GC_PH00243
ChiralDrugs
In many instances stereochemical properties of
chiral drugs are the controlling factor concerning
activity.One enantiomermight provide abiolog-
ical function, the other might be inactive or
might exhibit another functionality, which could
result in side effects. In some cases, one optical
isomer might be harmful. Enantiomeric separa-
tionof these compounds for accurate interpreta-
tion of drug tests, is easily achieved on Rt-
β
DEXcst™ and Rt-
β
DEXsm™ chiral capillary
GCcolumns.
30m, 0.25mm ID, 0.25µm
Rt-
β
DEXcst
™
(cat.# 13103)
Oven temp.:
120°C (hold 1min.) to
175°C@ 1.5°C/min.
Carrier gas:
helium
Linear velocity: 25cm/sec. set@ 120°C
Det.:
MSD set@ 220°C.
free
literature
AGuide to the Analysis of Chiral Compounds by GC
lit. cat.# 59889
Call Restek at 800-356-1688 or 814-353-1300, ext. 5, or contact
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