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4
6
8
10 min.
1
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5
0
2
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14 min.
1
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Feature
Supported by experienced
HPLC technical service.
Product of strictly controlled
in-housemanufacturing
Economical.
Benefit
Freemethod development assistance.
Column-to-column reproducibility;
fast order fulfillment.
Same peak shape as higher-priced
columns, but at a better value.
Features&Benefits
Pinnacle
II
™
Amino
HPLCColumns
Newest phase available in our line of
Pinnacle II
™
HPLCSilica
At-a-Glance
Product
Information
fromRestek
800-356-1688
814-353-1300
Sugar analysis is important in the generation of
nutrition panels, as both the sugar content and
the total carbohydrate contentmust be included.
Simple sugars includemono- and disaccharides
such as fructose, glucose, sucrose, maltose, and
lactose. In foods and beverages, they provide
sweetness, texture, and color development. The
perceived sweetness of each sugar is different in
terms of character, intensity, and duration.
Therefore, the ability to profile the individual
mono- and disaccharides is important to the
food chemist. Methods exist for the quantifica-
tion of individual sugar species, as well as for
the determination of total sugar content. The
new Pinnacle
II
™
Amino column is ideal for
these sugar analyses. HPLC analysis using an
amino-based stationary phase is themost popu-
lar technique for the routine analyses of simple
sugars. This analysis uses isocratic elution (e.g.,
water:acetonitrile, 25:75) and a refractive index
detector (RI). ReviewFigures 1 through3 to see
how the new Pinnacle
II
™
Amino column can
achieve good resolution of common sugars.
Another sugar, lactulose, is a common laxative
ingredient.The testmethod for this compound is
prescribed in the United States Pharmacopoeia
(USP 25). The assay calls for an analytical col-
umnwith an L8 (propylamino) stationary phase
bonded to 3µm particle silica, and use of refrac-
tive index detection. The new Pinnacle
II
™
Amino column allows excellent quantification
and peak shape for lactulose. Observe the lactu-
lose chromatogram analyzed per the USP
monograph in Figure 4.
LC_0202
PeakList:
1. fructose
2 glucose
3. sucrose
4. maltose
5. lactose
Sample:
Inj.:
5.0µL
Conc.: 15mg/mL each
Solvent: methanol:water
(10:90, v/v)
Column:
Pinnacle
II
™
Amino
Catalog #:
9217565
Dimensions: 150 x 4.6mm
Particle Size: 5µm
Pore Size:
110Å
Conditions:
Mobile Phase: water:acetonitrile
(25:75, v/v)
Flow:
1.0mL/min
Temp.:
35°C
Det.:
RI
Figure 1
5-Sugar Standard on Pinnacle
II
™
Amino
LC_0204
Figure 2
PureMaple Syrup on Pinnacle
II
™
Amino (high sensitivity)
PeakList:
1. fructose
2. glucose
3. sucrose
Sample:
Inj.:
100.0µL
Conc.:
1% puremaple
syrup in solvent
Solvent: acetonitrile:water
(70:30, v/v)
Column:
Pinnacle
II
™
Amino
Catalog #:
9217565
Dimensions: 150 x 4.6mm
Particle Size: 5µm
Pore Size:
110Å
Conditions:
Mobile Phase: water:acetonitrile
(25:75, v/v)
Flow:
1.0mL/min
Temp.:
35°C
Det.:
RI
LC_0205
1 2
3
0
2
4
6
8
PureMaple Syrup
on Pinnacle
II
™
Amino
(low sensitivity)
