Abstract
Mixtures of nucleotides, nucleosides and their respective
purine or pyrimidine bases are difficult to analyze by
reversedphase highperformance liquid chromatography
(RPHPLC). These compounds cover awide range of
polarities and functionalities, from the acidic nucleotides to
the basic purines and pyrimidines,making it very difficult
to retain and resolve all of themwith conventional alkyl
stationary phases. Traditional HPLC analyses of these
compounds often use a combination of reversed phase- ion
pairing (RP-IP) and/or ion exchange (IEX)mode.
