Abstract (cont.)
Nucleotides are often analyzed by anion exchangewhile
nucleosides are sometimes analyzedby cation exchange
modes. Thesemethods are not compatiblewith all the
solutes in thesemixtures and lack ruggedness.
This study is important in demonstrating that all three
classes of compounds (nucleotides, nucleosides, bases) can
be analyzedbyRP-HPLCusingone column and the same
simple isocraticmobile phase. This provides greater
convenience, reproducibilty and ruggedness in developing
methods for thesemixtures.
