RtxPresentations07 - page 426-427

HPLCConditions

• Column:Ultra IBD, 150x4.6mm, 5um (Restek

Corporation)

• Instrument: HP 1100

• Mobile Phase: 97.5:2.5 (v/v) 20mMAmmonium acetate,

pH 5.8:MeOH

• FlowRate: 1.0mL/min

• Detection:UV260nm

• Temperature: 35°C

• InjectionVolume: 20

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