HPLCConditions (cont.)
Samples:Mixtures of standards dissolved in 20mM
Ammonium acetate, pH 5.8. Concentrations of individual
componentswere approximately80-360
m
g/mL for the five
componentmixtures and approximately 40-160
m
g/mL for the
11 componentmixture.
All nucleotides had the phosphates linked to theC5 hydroxyl of
ribose (ie. 5'-ATP).
