restekapp07 - page 39

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UltraC4, 3µmColumns
Particle Size: 1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
3µm cat.# cat.# cat.# cat.#
30mm length 9102331 9102332 9102333 9102335
50mm length 9102351 9102352 9102353 9102355
100mm length 9102311 9102312 9102313 9102315
UltraC4, 3µmColumnswithTrident™ Inlet
Particle Size: 2.1mm ID 3.2mm ID 4.6mm ID
3µm cat.# cat.# cat.#
30mm length — 9102332-700 9102333-700 9102335-700
50mm length — 9102352-700 9102353-700 9102355-700
100mm length — 9102312-700 9102313-700 9102315-700
UltraC4, 5µmColumns
Particle Size: 1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
5µm cat.# cat.# cat.# cat.#
30mm length 9102531 9102532 9102533 9102535
50mm length 9102551 9102552 9102553 9102555
100mm length 9102511 9102512 9102513 9102515
150mm length 9102561 9102562 9102563 9102565
200mm length 9102521 9102522 9102523 9102525
250mm length 9102571 9102572 9102573 9102575
UltraC4, 5µmColumnswithTrident™ Inlet
Particle Size: 2.1mm ID 3.2mm ID 4.6mm ID
5µm cat.# cat.# cat.#
30mm length — 9102532-700 9102533-700 9102535-700
50mm length — 9102552-700 9102553-700 9102555-700
100mm length — 9102512-700 9102513-700 9102515-700
150mm length — 9102562-700 9102563-700 9102565-700
200mm length — 9102522-700 9102523-700 9102525-700
250mm length — 9102572-700 9102573-700 9102575-700
© Copyright 2000, Restek Corporation
column versus 3671mL for the competitor C4 column.
Mobile phase volumes were converted to column volumes
using the initial elution volumes for uracil (0.553 mL for
competitor C4, 0.538mL for Ultra C4). Following the 50th
gradient cycle, the columns were exposed to 8.5 hours of
continuous flow of 100%mobile phase A. The test columns
were exposed to a total of 54 gradient cycles.
Results
Figure 1 shows the percent change in capacity factor (k') of
phenylheptane plotted against the column volumes of the
TFA mobile phase. After approximately 3000 column
volumes of TFA, the competitor C4 column showed twice
the loss in retention as compared to the Ultra C4 column.
Even after 7000 column volumes, the Ultra C4 column lost
less than 10% of its original retention, while the competitor
C4 column showed a 44% loss in retention.
Conclusions
Restek’s Ultra C4 column combines high ligand density and
a unique bonding chemistry to attain excellent resistance to
acid hydrolysis. This resistance to stationary phase loss
ensures highly reproducible and consistent retention even
when using a very acidic mobile phase.
References
1. J.J.Kirkland, J.W.Henderson, J.J.Destefano,M.A.VanStraten,H.A.Claessens,
J. Chromatogr., A, 762 (1-2), 97-112 (1997).
2. J.J.Kirkland, J.L.Glajch, andR.D.Farlee, Anal.Chem. 61, 2-11 (1989).
References not available fromRestek.