Figure2
Allure
™
PFPPropyl column baseline separates cocaine (COC) and its metabolite,
ecgoninemethyl ester (EME), with excellent peak shape in less than fiveminutes.
0
1
2
3
4
5
6
7
8
9 min.
1
2
3
4
5
RestekCorporation • (800)356-1688 • (814)353-1300
Data Courtesy of Shane Needham, Pfizer Inc
Column:
Allure
™
PFPPropyl
Catalog#:
9169532
Dimensions:
30mmx2.1mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
5mMpH3.0
ammonium formate:
acetonitrile (10:90)
Flow:
0.6mL/min.
Column temp.:
ambient
Det.:
PE/SciexAPI 3000
Interface:
Turbo IonSpray,ESI
Interface temp.:
150°C
Ion mode:
positive
ESI probe voltage:
5000V
Orifice:
+/-71V
Ring:
+/- 265V
Collision gas:
Nitrogen
Collision gas pressure:
2.2mTorr
Collision gas energy(COC): 28eV
Collision gas energy(EME): 26eV
Electron multiplier:
2100 volts
Auxillary gas flow:
7000cc/min.
Nebulizer gas setting:
15lb/in.
2
Curtain gas setting:
12lb/in.
2
PeakList:
1. EME (ecgoninemethyl ester)
2.COC (cocaine)
SampleConditions:
Inj.:
10µL
Conc.:
1µg/mL
Solvent:
water
Temp.:
4°C
LC_0126
1
2
0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5 min.
XICof +MRM (3pairs) for 304.0 / 182.0 amu
2.35e3 cps
0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5 min.
XICof +MRM (3pairs) for 200.0 / 182.0 amu
1.30e3 cps
12x increase
inCOC response
over C18
LC_0137
PeakList:
1. cytosine
2. uracil
3. guanine
4. thymine
5. adenine
Sample:
Inj.:
1.0 mL
Conc.
100µg/mL
Solvent:
20mMAmmonium
Acetate,pH5.0.
Ammonium hydroxide
added to keep guanine
in solution
Column:
Allure
™
PFPPropyl
Catalog #:
9169565
Dimensions:
150 x 4.6mm
Particle Size: 5µm
PoreSize:
60Å
Conditions:
Mobile Phase: 20mMAmmonium
Acetate, pH5.0:
Methanol (85:15, v/v)
Flow:
1.0mL/min
Temp.:
ambient
Det.:
UV@260nm
Figure3
Nucleic bases as guanine, cytosine, uradine, thymine, and adenine are all easily retained upon the Allure
™
PFPPropyl.
Therefore, Restek’s Allure
™
PFP Propyl and Ultra PFP columns
are the best choice for the analysis of basic analytes. COC,
EME, quinine, morphine, beta-blockers, and tricyclic antide-
pressants have been analyzed successfully in urine, blood, and
tissue samples. In addition, pyridines, pyrimidines, and multi-
halogenated compounds have been successfully separated on
the Ultra PFP column.
The Allure
™
PFP Propyl and Ultra PFP columns provide
superior retention and peak shape for analytes having a pKa
>8 without the need for modifers, and they can provide
increased sensitivity for LC/MS ESI due to the high level of
organic solvent used in the mobile phase.
