RestekCorporation • (800)356-1688 •
Figure1
Evening primrose oil
30m, 0.25mm ID, 0.25µm FAMEWAX
™
(cat.# 12497).
Oven temp.:
165°C (hold 30min.) to 220°C@ 1.5°C/min.
(hold 15min.);
Inj. temp.:
225°C;
Det. temp.:
230°C;
Carrier gas:
Helium@ 40cm/sec.
1
40
1. C16:0
2. C18:0
3. C18:1 n-9
4. C18:2 n-6
5. C18:3 n-6
2
3
4
5
Figure2
Flax seedoil
30m, 0.25mm ID, 0.25µm FAMEWAX
™
(cat.# 12497).
Oven temp.:
165°C (hold 30min.) to 220°C@ 1.5°C/min.
(hold 15min.);
Inj. temp.:
225°C;
Det. temp.:
230°C;
Carrier gas:
Helium@ 40cm/sec.
1
1. C16:0
2. C18:0
3. C18:1 n-9
4. C18:2 n-6
6. C18:3 n-3
2
3
4
6
Results
Regarding the content of theOmega-3 andOmega-6 essential
fatty acids in the four oils studied, all containLA (18:2n6)
(
Figures 1-4andTable I
). Eveningprimrose oil (
Figure1
)
contains the largest amount of LA andwouldbe the best source.
Flax seedoil (
Figure 2
) reveals a significant amount of LNA
(C18:3n3), andwouldbe the best source of thisEFA.Onlyblack
currant seedoil (
Figure 3)
contains an approximately equal
amount of both isomeric linolenic acids.All chromatograms,
except that of flax seedoil, illustrate the presence ofGLA
(C18:3n6). In fact, borage oil (
Figure4
) andblack currant seed
oil are significant sources ofGLA.
Conclusion
Omega-3 (n-3) andOmega-6 (n-6)EFAs performkey functions
inour bodies.Quantifying these compounds innutritional
supplements such as eveningprimrose oil, flax seedoil, black
currant seedoil, andborage oil is successfully achievedusing the
FAMEWAX
™
column. This columnoffers excellent efficiency
and selectivity towards thesepolyunsaturatedmethyl esters,
providing an accuratedeterminationof the fatty acidprofiles.
Thus theFAMEWAX
™
column is an excellent column choice for
this and similar applications.
20
min.
40
20
min.
Achieve baseline resolution of complex polyunsaturated FAMEs in significantly less
timeusing theFAMEWAX
™
columnas compared toother Carbowax
®
columns!
Call 800-356-1688or 814-353-1300, ext. 3, or your local Restek representative toorder your FAMEWAX
™
column today.
