CLINICAL/FORENSICS
8
Basic Drugs (Mixed)
MixedBasicDrugs
Manybasic compounds canbe screened for in any given analysis, using a singleRestek column.Our
bondedpolysiloxane stationaryphases exhibit low columnbleed andhigh thermal stabilitywhile
offering a varietyof compositions that yield selective retention for specific analytes. Screening and
confirmation for individual drugs canbe performed simultaneouslybyusing twodissimilar station-
aryphases in adual column configuration.
Rtx
®
-5 andRtx
®
-35phases are 5% and 35%phenyl/methyl polysiloxanes, respectively. TheRtx
®
-5 col-
umnhasbeenusedas theprimaryanalytical columnbymany laboratories andcan resolveall 38com-
pounds in thismixture of basic drugs in less than 60minutes.The higher phenyl content of theRtx
®
-
35 phase increases its polarity and retention of more polar compounds, resulting in several elution
orderandretention time shifts.Under the sameconditionsused for theRtx
®
-5column, theRtx
®
-35col-
umn also resolves all 38basic drugs,with the exceptionof pyrilamine andbupivacaine.
Rtx
®
-200 is a trifluoropropyl polysiloxane that exhibits unique selectivity for compounds with lone
pairs of electrons. This columndisplays verydifferent retention times and elutionorders in compari-
son to the Rtx
®
-5 or Rtx
®
-35 column. Under the same analytical conditions, several coelutions occur
with the Rtx
®
-200 column, including: cotinine/caffeine, bupivacaine/chlorpromazine,
bromazepam/prazepam, and flurazepam/papaverine.
Because all three columns canbeoperatedunder the same temperatureprogram conditions, simulta-
neous dual column confirmation analysis canbe accomplishedby using either anRtx
®
-35 columnor
anRtx
®
-200 column in conjunctionwithanRtx
®
-5 column.This increases qualitative andquantitative
reliabilitywithout sacrificing analysis time.
GC
1. nicotine
2. benzocaine
3. cotinine
4. meperidine
5. caffeine
6. benzphetamine
7. ketamine
8. diphenhydramine
9. lidocaine
10. phenyltoloxamine
11. tripelennamine
12. phenothiazine
13. dextromethorphan
14. methadone
15. amitriptyline
16. trimipramine
17. tetracaine
18. pyrilamine
19. medazepam
20. bupivacaine
21. scopolamine
22. codeine
23. morphine
24. diazepam
25. chlorpromazine
26. temazepam
27. flunitrazepam
28. bromazepam
29. prazepam
30. acetopromazine
31. flurazepam
32. papaverine
33. clonazepam
34. haloperidol
35. alprazolam
36. triazolam
37. thioridazine
38. trazodone
min.
10
20
30
40
50
60
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
21 20
22
24
25
26
28
27
29
30
31
32
23
33
35
36
37
34
16
17
18
19
GC_PH00235
30m, 0.25mm ID, 0.25µmRtx®-5 (cat.# 10223)
1.0µL split injection of a basic drug sample.
Conc.:
1000ng/µL
Oven temp.:
100°C to 325°C@ 4°C/min. (hold 10min.)
Inj. / det. temp.:
250°C / 320°C
Carrier gas:
helium
Linear velocity:
30cm/sec. set@ 100°C
FID sensitivity:
1.28 x 10
-10
AFS
Split ratio:
50:1
38
MixedBasicDrugs (Underivatized)
Rtx
®
-5
free
literature
ImprovedGCAnalysis of Basic
Organic CompoundsUsing
BaseDeactivatedColumns&
Liners.
lit. cat.# 59108
Call Restek at 800-356-1688
or 814-353-1300, ext. 5, or
contact your Restek
representative, to request
your free copy!
