CLINICAL/FORENSICS
4
Abused Inhalants; Anesthetics
GC
Abused Inhalants
Rtx®-BAC1&Rtx®-BAC2
30m, 0.32mm ID, 1.80µmRtx
®
-BAC1 (cat.# 18003)
30m, 0.32mm ID, 1.20µmRtx
®
-BAC2 (cat.# 18002)
Oven temp.:
40°C (hold 4min.) to 120°C@ 10°C/min.
Carrier gas:
helium
Linear velocity:
50cm/sec.
Detector:
FID
Sample:
250µL headspace
Transfer line temp.:
125°C
Sample loop temp.:
125°C
Vial temp.:
70°C
min.
1
2
3
4
5
6
7
8
9
10
11
1
Rtx®-BAC1
2
3
4
5
6
7
8
9
10/11
12
13
14
15
Acknowledgement: Analyses performed using aTekmar 7000Headspace
Autosampler on loan courtesy ofTekmar-Dohrmann.
GC_PH00446
1
2
3
4
5
6
7
8
9,11
10
12
13
14
15
Rtx®-BAC2
min.
1
2
3
4
5
6
7
8
9
10
11
Abused Inhalants/Anesthetics
Inhalant abuse can be detected during screening of whole blood, serum, or urine samples, using headspace (GC) with flame
ionizationdetection (FID).Here,weusedaGC equippedwithanautomatedheadspace sampler that simultaneously introduc-
es a sample into two analytical columns. A dual-column configuration provides screening and confirmational data from the
same injection.We used anRtx
®
-BAC1 column (30m, 0.53mm ID, 3.00µmdf) and anRtx
®
-BAC2 column (30m, 0.53mm ID,
2.00µmdf)—columns typicallyused in combination as a screening and confirmation column set for blood alcohol analysis.A
useful extensionof blood alcohol analysis using this column set is the detectionof other volatile organic compounds (VOCs),
such as those in inhalants and anesthetics.
GC
1. isoflurane
2. enflurane
3. halothane
4. methoxyflurane
30m, 0.53mm ID, 3.0µmRtx
®
-BAC1 (cat.# 18001)
30m, 0.53mm ID, 2.0µmRtx
®
-BAC2 (cat.# 18000)
1.0mL headspace sample
Oven temp.:
40°C (hold 5min.) to 240°C@ 5°C/min.
Inj. & det. temp.:
240°C
Carrier gas:
helium
Linear velocity:
65cm/sec.
AbusedAnesthetics
Rtx®-BAC1&Rtx®-BAC2
GC_PH00501
GC_PH00502
1. methanol
2. ethanol
3. diethyl ether
4. isopropanol
5. methylene chloride
6. acetone
7. methyl
tert
-butyl ether
8. hexane
9. chloroform
10. ethyl acetate
11. methyl ethyl ketone
12. benzene
13. trichloroethylene
14. toluene
15. methyl isobutyl ketone
Rtx®-BAC2
for
more
info
GCAnalysis of Commonly
Abused Inhalants inBlood,
UsingRtx
®
-BAC1 and
Rtx
®
-BAC2Columns
lit. cat.# 59548
A Technical Guide for Static
HeadspaceAnalysis, UsingGC
lit. cat.# 59895A
Call Restek at 800-356-1688 or
814-353-1300, ext. 5, or con-
tact your Restek representa-
tive, to request your free copy!
Rtx®-BAC1
