CommonlyAskedQuestions
•
Why
is the Ultra IBD different from other HPLC columns?
TheUltra IBD phase incorporates a polar functional groupwithin the
hydrocarbon chain, resulting in a column that exhibits excellent peak
shape for acids, bases, zwitterions, and neutral compounds. The unique
chemistry of this phase allows compounds to be retained by both
hydrophobic and polar interactions.
•
What advantage does the Ultra IBD offer for LC/MS analyses?
Mobile phaseswith high aqueous content are required to obtain adequate
retention of polar analyteswithmost conventional reverse phase
packings. However, mobile phaseswith high aqueous content can cause
sensitivity loss inLC/MS analyses. The polar interaction of theUltra
IBD phase provides excellent retentionwith higher organic content
mobile phases, and also greatly reduces or eliminates the need for
mobile phasemodifiers that are often required to obtain symmetrical
peaks and/or sufficient retentionwhen analyzing highly polar com-
pounds with other columns. Thismakes theUltra IBDmuchmore
compatiblewithMS detection than traditional straight chain alkyl
stationaryphases.
•
What are the particle and pore sizes of the silica used for the
Ultra IBD phase?
TheUltra IBD
is offered in both 3 and 5µm, spherical Type B silica
with a 100Angstrom pore size.
•
What are the pH and temperature limits for the Ultra IBD
phase?
TheUltra IBD phase can be used from pH 2 to pH 8 and at tempera-
tures up to 80
o
C.
•
Are guard columns available for the Ultra IBD columns?
Restek’s uniqueTrident
™
guard column system is available for theUltra
IBD
column. The integral leak-free design of the Trident
™
system
contains a cap frit that can be replaced easilywithout disturbing the
packed bed. The guard cartridge, packedwith theUltra
IBD phase also
canbe replaced easily as needed.
At-a-Glance
Product
Information
fromRestek
Ultra IBD
Restek is your free
technical literature source!
Application Notes:
(#59511) Improved HPLC
Analysis of Analgesics
(#59512) The Ultra IBD Column Allows
HPLC Separation of Polar and Non-Polar
Analytes from the Same Sample
(#59510) HPLC Stationary Phase Selection
for the Analysis of Steroids
(#59118) Allure
™
PFP Propyl HPLC Column
Provides Improved LC/MS Analyses of
Basic Compounds
(#59141) Analyze Nucleotides,
Nucleosides, Purine, and Pyrimidine Bases
Simultaneously with the Ultra IBD Column
(#59398) Analysis of Preservatives
Using HPLC
•
Call
814-353-1300 or
800-356-1688, ext. 5
•
Fax
814-353-1309
•
Online
800-356-1688
814-353-1300
