Ultra IBD
HPLCColumns
Excellent PeakShape forAcids andBases
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At-a-Glance
Product
Information
fromRestek
Feature
Separates acids, bases,
zwitterions, and neutral
compounds.
Can be usedwith awide range
ofmobile phase polarity and pH.
Excellent batch-to-batch
consistency.
Highly retentive phase.
Benefit
One column can analyze a
wide range of compounds
simultaneously.
More flexibility withmethod
development.
Reproducible analyses.
Improved LC/MS sensitivity
andHPLC resolution.
Features & Benefits
800-356-1688
814-353-1300
Restek’sUltra IBD (intrinsically based deactivated) phase exhibits
excellent peak shape and selectivity for acids, bases, zwitterions, and
polar compounds. In contrast to conventional C18 columns, theUltra
IBD phase contains a polar functional groupwithin the hydrocarbon
bonded chain. This allows the column to retain solutes not only by
hydrophobic interactions, but also by polar attraction forces between
the stationary phase and the analyte.
Restek’sUltra IBDcolumn
TheUltra IBD can operate under
both reverse and normal phase
conditions and retains compounds
across awide range ofmobile
phase compositions. Request a
copyofRestek’sApplications
Note cat. #59512,
The Ultra IBD
ColumnAllowsHPLC Separation
of Polar and Non-Polar Analytes
from the Same Sample
, for more
detailed information.
Visit our website
at
and see our
growing compilation of
HPLC application chromatograms:
LC_0129
Adenine, Adenosine, ATP,
ADP, and AMP on Ultra IBD
LC_0130
Cystosine, Cytidine, CTP,
CDP, and CMP on Ultra IBD
LC_0131
Guanine, Guanosine, GTP,
GDP, and GMP on Ultra IBD
LC_0132
Thymine, Thymidine, TTP,
TDP, and TMP on Ultra IBD
LC_0130
Uracil, Uridine, UTP, UDP,
and UMP on Ultra IBD
Figure1a
Restek’s Ultra IBD column exhibits excellent peak shape for acidic
and basic compounds at a neutral pH.
LC_0056
Peak List:
Conc.(µg/mL)
1. uracil
5
2. benzoicacid 50
3. maleate
5
4. trimipramine 50
5. nortriptyline 50
6. amitriptyline 50
Sample:
Inj.:
10µL
Column:
Ultra IBD
Catalog #:
9175565
Dimensions:
150 x 4.6mm
ParticleSize:
5µm
Pore Size:
100Å
Conditions:
MobilePhase:
20mM K
x
H
y
PO
4
pH7:acetonitrile
(65:35, v/v)
Flow:
1mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
0
5
10
15
20
25 min.
1
6
4
3
2
5
