Aldehydes/Ketones
DNPH Standard
ForUSEPAMethods TO-11Aand 8315
At-a-Glance
Product
Information
fromRestek
800-356-1688
814-353-1300
acetaldehyde-DNPH
acetone-DNPH
acrolein-DNPH
benzaldehyde-DNPH
n
-butyraldehyde-DNPH
crotonaldehyde-DNPH
2,5-dimethylbenzaldehyde-DNPH
formaldehyde-DNPH
hexaldehyde-DNPH
isovaleraldehyde-DNPH
propionaldehyde-DNPH
m
-tolualdehyde-DNPH
o
-tolualdehyde-DNPH
p
-tolualdehyde-DNPH
valeraldehyde-DNPH
Aldehyde-Ketone-DNPH TO-11A
CalibrationMix
(15 components)
15µg/mL* each in acetonitrile, 1mL/ampul
Each
31808
HPLCColumns
Ultra C18 5µmColumn
cat.#:
9174565
150 x 4.6mm
Additional Information
Formaldehyde andOther Aldehydes
Committee on Aldehydes, Board of Toxicology and
Environmental Hazards, National ResearchCouncil,
National Academy Press, Washington, DC, 1981.
For a complete listing of
RestekHPLC columns,
packings, and accessories, request the
2004RestekChromatography Products
catalog (lit. cat.# 59854).
This catalog details our
extensive product line and includes
hundreds of chromatograms.
• Convenient 15µg/mL concentration as aldehyde/ketone, similar to the
concentration of interest inmost ambient air work.
• Certificate ofAnalysis lists both aldehyde/ketone and -DNPH derivative
concentrations.
• Fast analysis using a RestekUltra C18HPLC column .
Carbonyl compounds, including lowmolecularweight aldehydes and ketones, are
receiving increased attention by the regulatory community.
Formaldehyde is the target compound forUSEPACompendiumMethodTO-11A
andMethod 8315, but at least 14 other carbonyl compounds also can be detected and
quantified.MethodT0-11Amodifies the sampling procedures outlined in earlier
MethodTO-5; the newermethod is based on the specific reaction between carbonyl
compounds and the 2,4-dinitrophenylhydrazine (DNPH) coating on a silica gel adsor-
bent (packed in cartridges), in the presence of a strong acid catalyst. The reaction pro-
duces stable, colored hydrazone derivatives.
Tomeet the needs of analystsmonitoring these compounds, Restek offers a new 15-
component aldehyde standard forUSEPACompendiumMethodTO-11A and
Method 8315. The concentration of themix components, 15µg/mL each, is similar to
the concentration of interest inmost ambient airwork. For convenience, our
Certificate ofAnalysis lists concentrations for both the aldehydes/ketones and the
DNPH derivatives.
A 150 x 4.6mmUltraC18HPLC column (cat.# 9174565) is optimal for fast, reliable
analyses of the derivatives of formaldehyde, other aldehydes, and ketones.
Fast, reliable analyses of aldehydes and ketones on Restek’s
Ultra C18 HPLC column.
2
4
6
8
10
12
14min.
LC_0036
1
2
4
5
7
8
3
6
9
10
11
12
13
DNPH derivatives of
1. formaldehyde
2. acetaldehyde
3. acetone
4. acrolein
5. propionaldehyde
6. crotonaldehyde
7. MEK
8. methacrolein
9. butyraldehyde
10. benzaldehyde
11. valeraldehyde
12.
m
-tolualdehyde
13. hexanaldehyde
Column:
Ultra C18
Catalog #:
9174565
Dimensions:
150 x 4.6mm
Particle size: 5µm
Pore size:
100Å
Conditions:
Mobile phase:
Solvent A: water
Solvent B: acetonitrile
Solvent C: THF
Gradient: 0-15min.
50-30%A
30-65%B
20-5%C
Flow rate:
1.5mL/min.
Wavelength:
365nm
Temp.:
40°C
Sample:
Inj.:
10µL
Conc.:
3.0µg/mL as derivative
formaldehyde-DNPH
Formaldehyde-DNPHMix
500µg/mL in acetonitrile, 1mL/ampul
Each
5-pk.
31837
31837-510
