Allure
™
C18
HPLCColumns
MaximumRetention andPeak Symmetry
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At-a-Glance
Product
Information
fromRestek
Feature
Highly base-deactivated silica.
Unique bonding technology
resists hydrolysis of bonded
phase.
Excellent batch-to-batch
consistency.
High carbon load phase.
Benefit
Excellent peak symmetry for basic
compounds.
More consistent retention times
and longer column lifetime.
Reproducible analyses.
Improved LC/MS sensitivity
andHPLC resolution.
Features & Benefits
Allure
™
C18 high carbon
load phase is ideal for
LC/MS applications
800-356-1688
814-353-1300
The sensitivity of LC/MS analyses
is strongly affected by the percentage of
organic content in the mobile phase.
High organic content can greatly
increase LC/MS signal-to-noise ratio.
However, most commercial C18 phases
containonly10 to15%carbon loadso that
lessorganicmustbeusedinthemobilephase
to retain solutes. TheAllure
™
C18 phase
has a 27% carbon load, which results in
increased retention and increasedLC/MS
sensitivity, evenwithhighorganicmobile
phases. In theLC/MSanalysisof steroids,
theAllure
™
C18 column shows a26% in-
crease in sensitivity when compared to a
conventionalC18phase (Figure2).
Figure1
Allure
™
C18separatesallUSEPAMethod8330explosives inunder 10minutes.
Restek’sAllure
™
C18 column is designed using a simple yet unique bonding
chemistry that offers improved resistance to hydrolysis and superior batch-
to-batch reproducibility. The high carbon load phase exhibits maximum
retention characteristics, making it ideal for liquid chromatography/mass
spectrometry (LC/MS) applications. The phase is bonded onto a highly
base-deactivatedType B silica, resulting in improved peak symmetry, even
for basic analytes. TheAllure
™
C18 column can be used to analyze awide
range of compounds, from steroids and antibiotics to explosives (Figure 1).
Peak List:
1. HMX
2. RDX
3. 1,3,5-trinitrobenzene
4. 1,3-dinitrobenzene
5. tetryl
6. nitrobenzene
7. 4-amino-2,6-dinitrotoluene
8. 2-amino-4,6-dinitrotoluene
9. trinitrotoluene
10. 2,6-dinitrotoluene
11. 2,4-dinitrotoluene
12. 2-nitrotoluene
13. 4-nitrotoluene
14. 3-nitrotoluene
Sample:
Inj.:
5µL
Conc.: 50µg/mL
Solvent: water:methanol (1:1)
0
2
4
6
8
10 min.
1
2
5
6
7
8
9
10
11
12
13
14
4
3
Column:
Allure
™
C18
Dimensions: 150 x 4.0mm
ParticleSize: 5µm
Pore Size:
60Å
Conditions:
MobilePhase: water:methanol
(45:55, v/v)
Flow:
1.0mL/min.
Temp.:
45°C
Det.:
UV@ 254nm
LC_0065
Figure2
Allure
TM
C18ColumnsShowa
26% Increase inLC/MSSensitivity for
SlightlyPolarSteroidsCompared to
ConventionalC18
1
2
3
0
1
2
3
4min.
Column:
Catalog#:
9164552
Dimensions:
50x2.1mm
Particlesize:
5µm
Poresize:
60Å
Conditions:
Scanrange:
320-520amu
Flowrate:
0.4mL/min.
LC/MSInterface: Positive ion
ESI-TOF
Voltage:
3800V
Nozzletemp.:
160V
Nozzlevolt.:
190V
PeakList
1. deoxycorticosterone
glucoside
2. deoxycorticosterone
3. deoxycorticosterone
acetate
LC_0111
Data Courtesy of Shane Needham, Pfizer Inc
Allure
™
C18
H
2
O:MeOH(33:67)
4191.1cps
ConventionalC18
H
2
O:MeOH(40:60)
3324.0cps
