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Figure 3 Sharp, easily differentiated organic acid profiles for
cranberry juice cocktail on an Allure® Organic Acids column.
Figure 2 Excellent resolution of organic acids, including tartaric
and quinic acids, on an Allure® Organic Acids column.
• 11 • 2007 vol. 2
Foods, Flavors & Fragrances
In analyses of organic acids, specifically, under
high aqueous mobile phase conditions, the
Allure® Organic Acids column is the column of
have developed amethod using a 300mm
Allure® Organic Acids column to separate critical
organic acids: tartaric, quinic, malic, citric and
fumaric acids. This method calls for 100% aque-
ous mobile phase as recommended by AOAC
method 986.13. The Allure® Organic Acids column
is tested specifically for resolving critical organic
acids. Figure 2 shows that tartaric and quinic acids
are resolved to baseline; Figure 3 shows typical
analyses under the conditions we recommend.
Peak List: Conc. (mg/mL)
1. tartaric acid 1
2. quinic acid 1
3. malic acid 1
4. citric acid 1
5. fumaric acid 0.005
Sample:
Inj.: 10μL standard solution
Solvent: water
Column: Allure® Organic Acids
Cat. #: 9165585
Dimensions: 300 x 4.6mm
Particle size: 5μm
Pore size: 60Å
Conditions:
Mobile phase: 100mM phosphate buffer, pH 2.5
Flow: 0.5mL/min.
Temp.: ambient
Det.: UV @ 226nm
LC_0238
Sample:
Inj.: 10μL
Conc.: cranberry juice:water (50:50, v/v)
Solvent: water
Column: Allure® Organic Acids
Cat. #: 9165585
Dimensions: 300 x 4.6mm
Particle size: 5μm
Pore size: 60Å
Conditions:
Mobile phase: 100mM phosphate buffer, pH 2.5
Flow: 0.5mL/min.
Temp.: ambient
Det.: UV @ 226nm
LC_0236
Peak List:
2. quinic acid
3. malic acid
4. citric acid
References
1.
2. Official Methods of Analysis (2000). AOAC International, 17th edition,
method # 986.13.
3. Manolaki, P. et al., Food Chemistry, 98 (2006), page 658-663.
4. Kafkas, E. et al., Food Chemistry, 97 (2006), page 732-736.
Allure® Organic Acids Column
5μm Column, 4.6mm cat. # price
150mm 9165565 $529
ordering note
For guard cartridges for these columns, visit our website at
.
citric acid 2000μg/ml
fumaric acid 10*
malic acid 2000
quinic acid 2000
tartaric acid 2000
Fruit Juice Organic Acid Standard
In water, 1mL/ampul
cat. # 35080 (ea.) $24
In water, 5mL/ampul
cat. # 35081 (ea.) $38
for more info
For more information on our Allure® Aqueous C18, Ultra Aqueous
C18 and Allure® Organic Acid columns, visit us online at
.
5μm Column, 4.6mm cat. # price
150mm 9178565 $385
Ultra Aqueous C18 Column (USP L1)
Allure® Aqueous C18 Column
5μm Column, 4.6mm cat. # price
150mm 9168565 $385
2
3
4
*Fumaric acid is a trace impurity in malic acid, as well as an
added component of the mix. The amount of fumaric acid in
malic acid will not affect the stated concentration of malic acid,
but can represent a significant and variable deviation from the
low concentration of fumaric acid stated to be in the mix. All
other components of the mix are at the specified concentration.
Get More!
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