ADV01-05_1-298

97 / 298

Figure 4
A Pinnacle II™ C18 column resolves all 5 components of a BTEX standard in 15 minutes.
• 3 •
for moreinfo
request the Pinnacle™ II product flyer
(lit. cat.# #59281).
LC_0161
Environmental Applications
Pinnacle™ II HPLC columns provide a cost-effective
tool for many traditional methods used in the envi-
ronmental industry, including US Environmental
Protection Agency (EPA) Methods 8310 and 8330, as
well as methods for analyzing BTEX (Figure 4) and
carbamates.
Restek controls Pinnacle™ II raw material quality
from the very beginning of the silica manufacturing
process. Add our phase bonding and column pack-
ing experience to this high level of quality control,
and you benefit from even better column-to-column
and analysis-to-analysis reproducibility.
Peak List:
1. benzene
2. toluene
3. o-xylene
4. ethylbenzene
5. m-xylene & p-xylene
Sample: BTEX standard (cat.# 30213)
was diluted 1:10
Inj.: 10µL
Conc.: 200µg/mL
Solvent: acetonitrile
Column: Pinnacle II C18
Catalog#: 9214565
Dimensions: 150x4.6mm
Particle size: 5µm
Pore size: 110Å
Conditions:
Mobile phase: water:acetonitrile (50:50, v/v)
Flow: 1.0mL/min.
Temp.: ambient
Det.: UV @ 254nm
✔ Pinnacle II™ C8 5µm Columns
Length 1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
50mm 9213551 9213552 9213553 9213555
100mm 9213511 9213512 9213513 9213515
150mm 9213561 9213562 9213563 9213565
250mm 9213571 9213572 9213573 9213575
✔ Pinnacle II™ C18 5µm Columns
Length 1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
50mm 9214551 9214552 9214553 9214555
100mm 9214511 9214512 9214513 9214515
150mm 9214561 9214562 9214563 9214565
250mm 9214571 9214572 9214573 9214575
✔ Pinnacle II™ Phenyl 5µm Columns
Length 1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
50mm 9215551 9215552 9215553 9215555
100mm 9215511 9215512 9215513 9215515
150mm 9215561 9215562 9215563 9215565
250mm 9215571 9215572 9215573 9215575
✔ Pinnacle II™ Cyano 5µm Columns
Length 1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
50mm 9216551 9216552 9216553 9216555
100mm 9216511 9216512 9216513 9216515
150mm 9216561 9216562 9216563 9216565
250mm 9216571 9216572 9216573 9216575
Many New Phases Coming Soon!
Check our web site for additional stationary phases in this family of products.
.High backpressure is one of the most
common problems encountered when
performing HPLC analysis. Normal
column backpressure is observed after
a new column has been installed and
equilibrated with the mobile phase.
Unfortunately, this pressure will often
increase with use because of particles
collecting on the column inlet frit.
These particles can be sample impuri-
ties, mobile phase contaminants, or
materials from the injector or
autosampler rotor seal. The presence
of particles can result in increased
backpressure, split peaks, tailing, and
eventually over-pressure shut-down. In
some circumstances, these problems
can be corrected by back-flushing the
column. However, in many cases the
result is an unusable column.
To reduce backpressure problems, all
samples and mobile phase solvents
must be filtered before use, and rotor
seals should be changed on a routine
basis. Along with these preventative
measures, it is advisable to use column
prefilters such as the Trident™ Direct
column protection system. Particles
build up on the inexpensive, replace-
able frit in the prefilter, instead of the
permanent frit at the head of the
column.
For more information on the Trident™
Direct guard column system and a
complete product offering, request lit.
cat.# 59314.
Hot Tech Tip