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Pinnacle II™
New HPLC Columns, cont.
Column: Pinnacle II C18
Catalog#: 9214565
Dimensions: 150x4.6mm
Particle size: 5µm
Pore size: 110Å
Conditions:
Mobile phase: A: 1% acetic acid in water
B: acetonitrile
Time (min.): % B
0 50
2 50
9 80
12 80
13 50
Flow: 1.0mL/min.
Temp.: ambient
Det.: UV @ 280nm
Peak List:
1. nordihydrocapsaicin
2. capsaicin
3. dihydrocapsaicin
Sample: habanero nugget extract
Inj.: 10µL
Conc.: 4000µg/mL
Solvent: ethanol
0 1 2 3 4 5 6 7 min.
1
2
3
Figure 3
The Pinnacle II ™ C18 column achieves good resolution of three target capsaicinoids.
LC_0160
Nutraceutical and Food Applications
The Institute for Nutraceutical Advancement (INA)
has published a series of methods for the determina-
tion of active compounds in nutraceutical products.
Pinnacle II™ HPLC columns exhibit excellent peak
shapes and reproducibility for natural product analy-
ses (Figure 2).
As society's taste in spicy foods grows, so does the
need to test and classify products and raw materials
for their heat levels. AOAC Method 995.03 specifies
the separation of the three target capsaicinoids
responsible for the heat in chili peppers. Using a gra-
dient elution program, an efficient separation of
these compounds is achieved with the Pinnacle II™
C18 column (Figure 3).
Figure 2
The Pinnacle II™ C18 column achieves excellent separation of hyperforin and adhyperforin
when analyzing a St. John’s Wort capsule.
Exclusive!
Restek offers the
Trident Direct™ guard cartridge system.
All of our Pinnacle™ II phases are available
in guard columns. The Trident Direct™
system features three levels of protection
against many of the contaminated samples
that analysts inject into a system.
Many New Phases Coming Soon!
Additional stationary phases will be
joining this family of products.
Base-Deactivated Columns Coming
Soon!
A line of columns that offer the structural
integrity of Type A silica, combined with
an improved treatment for base
deactivation. Excellent for analyzing
basic compounds.
Restek also offers a full line of HPLC
accessories—everything from
PEEK ™ fittings to syringes.
Peak List:
1. hyperforin
2. adhyperforin
Sample: St. John's Wort capsule
Inj.: 20µL
Conc.: 4170µg/mL
Solvent: methanol
Column: Pinnacle II C18
Catalog#: 9214565
Dimensions: 150x4.6mm
Particle size: 5µm
Pore size: 110Å
Conditions:
Mobile phase: acetonitrile:0.01N
phosphate buffer, pH 2.5
(15:85, v/v)
Flow: 1.2mL/min.
Temp.: 30°C, autosampler at 15°C
Det.: UV @ 270nm
LC_0175