Chromatography Columns 2013+ - page 333

Ultra II®Biphenyl (ESI+)
Column Ultra II® Biphenyl (cat.# 9609352)
Dimensions: 50mm x 2.1mm ID
Particle Size: 3µm
Temp.: 40°C
Sample
Conc.: 50 ng/mL,
diluted 10x inmobile phase
Inj. Vol.: 5µL
Mobile Phase A: 0.1% formic acid inwater
B: 0.1% formic acid inmethanol
Time (min.) Flow (mL/min.) %A %B
0 0.5 90 10
6.00 0.5 0 100
6.1 0.5 90 10
8.00 0.5 90 10
LC_CF0509
1. Amiloride
2. Triamterene
3. Indapamine
4. Ethacrynic acid
5. Bumetanide
Excellent retention for
highly functionalized
compounds!
Ultra II®Biphenyl (ESI-)
1. Acetazolamide
2. Chlorothiazide
3. Hydrochlorothiazide
4. Bendroflumethiazide
5. Furosemide
LC_CF0510
PainManagementDrugs inUrine
Sample
Conc.: 50 ng/mL, diluted 10x in
mobile phase
Inj. Vol.: 5µL
Mobile Phase
A: 0.1% formic acid inwater
B: 0.1% formic acid inmethanol
Time (min.) Flow (mL/min.) %A %B
0 0.5 90 10
10 0.5 0 100
10.1 0.5 90 10
12 0.5 90 10
Peaks
1. Acetaminophen
2. Morphine
3. Codeine
4. Oxycodone
5. Hydrocodone
6. Fentanyl
7. Buprenorphine
8. Lorazepam
9. Diazepam
10. Methadone
LC_CF0516
3 visit www.restek.com/biphenyl
Phenomenex®Gemini®C6-Phenyl
• Poor retention, inadequate
resolution frommatrix.
• Isobaric interferencesprevent
quantification.
3μmPhenomenex®
Gemini®C6-Phenyl
50mm x2.0mm
(Same conditionsasUltra II® Biphenyl.)
Ultra II®Biphenyl (ESI+)
Fast, reliable resolutionof target
compounds.
Improved retention reduces risk
ofmatrixeffects.
3μmUltra II®Biphenyl
50mm x2.1mm
(cat.#9609352)
Detector Applied Biosystems/MDS Sciex LC/MS/MS, Model#API 5000™
Acknowledgement Special thanks to Applied Biosystems for providing instrument time.
Detector Applied Biosystems/MDS Sciex LC/MS/MS,
Model#API 5000™
Acknowledgement Special thanks to Applied Biosystems for
providing instrument time.
Diuretics
Formore informationon reducingmatrixeffects, visit www.restek.com/adv005
Website :www.chromtech.net.auE-mail: info@chromtech.net.auTeNo: 03 9762 2034 . . . inAUSTRALIA