Biphenyl columns are unique as they combine the performance of a traditional alkyl phase (C8 orC18)
with that of a phenyl phase, providing unmatched versatility formethod developers. Extensive research
went into creating this new phase, which offers excellent retention for both polar and nonpolar com-
pounds.Thebondingof twophenyl groups end-to-end iswhatmakesBiphenyl columnsunique in struc-
ture and trulyunparalleled inperformance.Rather thanusing a straight chainhexyl linker, theBiphenyl
phase incorporates an aryl linker, making it more hydrophobic than conventional phenyls. The overall
result is a phase that offers the highest degree of aromatic selectivity and hydrophobic retention of any
phenyl phase. Maximize method development success with versatile Biphenyl columns—
no other
phasecanprovidebothC18-andphenyl-likeperformance inasinglecolumn.
visit
2
Ultra II®Biphenyl Columns
GreaterVersatility—IncreasedMethodPerformance
Amphetamines
(LC/MSESI+)
Columns
Ultra II
®
Biphenyl (cat.# 9609552)
Conventional C18
Dimensions:
50mm x 2.1mm ID
Particle Size:
5µm
Pore Size:
100 Å
Temp.:
30 °C
Sample
Diluent:
mobile phase
Conc.:
2µg/mL
Inj. Vol.:
5µL
Mobile Phase
A: 0.1% formic acid inwater
B: 0.1% formic acid in
methanol
Time (min.) Flow (mL/min.)
%A
%B
0
0.3
80
20
10
0.3
5
95
10.1
0.3
80
20
12.0
0.3
80
20
Ultra II Biphenyl
Conventional C18
Peaks
RT (min.)
RT (min.)
1. Amphetamine
2.812
2.725
2. Methamphetamine 3.432
2.763
3. MDA
3.537
2.788
4. MDMA
4.092
2.808
5. MDEA
4.679
3.261
LC_CF0518
• Resolveawider rangeof compoundchemistries compared toaC18
orphenyl column.
• Easy separationswithmethanolicmobilephases—noacetonitrileneeded.
• Perfect fordrugsof abuse, pharmaceuticals,metabolitesandmore.
• Enhancedandalternate selectivity—ideal formethoddevelopment and
column switching.
Si
BIPHENYL
ConventionalC18
LC_CF519
Ultra II®Biphenyl
Enhanced selectivityvs. C18.
Improvedpeak shape—noharsh
modifiers.
Website :
:
: 03 9762 2034 . . . inAUSTRALIA
