ParabensonUltra II™C18
2
•
800-356-1688
C18
Si
CH
3
CH
3
O
AqueousC18
Si
OO
P
Silica
Si
OO O
OH
Biphenyl
Si
CH
3
CH
3
O
PROPRIETARY
Aromax
PFPPropyl
Si
CH
3
CH
3
O
F
F
F
F
F
Ultra II™AromaxColumns (USPL11)
Ultra II Aromax is a unique reversed phase material that
exhibits superior retention and selectivity for aromatic
and/or unsaturated compounds, compared to conventional
alkyl andphenyl phases.This column is agreat alternative to
our Biphenyl phase when increased retention is required. A
very suitable choice for analysis of steroids, tetracyclines,
drugmetabolites, and other compounds that contain some
degree of unsaturation.
Ultra II™BiphenylColumns (USPL11)
A unique reversed phase material that exhibits both
increased retention and selectivity for aromatic and/or
unsaturated compounds, compared to conventional alkyl
and phenyl phases. This is a great alternative to a C18
column when alternative selectivity is desired. An excellent
choice for the analysis of steroids, tetracyclines, drug
metabolites, andother compounds that contain somedegree
of unsaturation.
Ultra II™C18Columns (USPL1)
A retentive, highly purematerial that exhibits excellent peak
shape for a wide range of compounds. This is a robust and
very reproducible general-purpose reversedphase column.
Ultra II™AqueousC18Columns (USPL1)
Highly retentive and selective for reversedphase separations
of polar analytes.Highly base-deactivated. Compatiblewith
highly aqueous (up to100%)mobile phases.
Ultra II™PFPPropylColumns (USPL43)
A pentafluorophenyl phase with a propyl spacer. Highly
retentive for basic analytes.An excellent phase for separating
nucleosides, nucleotides, purines, pyrimidines, and halo-
genated compounds.
Ultra II™SilicaColumns (USPL3)
High surface area.TypeB silicapacking.
TheUltra II™ product line is the first LC column line specifically designed
foruniversal application—optimal chromatographyonany system.This col-
umn line is built on a highly inert, high surface area silica that is completely
Restekmanufactured, providing excellent column-to-column reproducibili-
ty.OnlyUltra II™ columns are available in a comprehensive range of parti-
cle sizes (1.9µm, 2.2µm, 3µm, and 5µm), creating truly scalable chromatog-
raphy on any type of LC instrument, from conventional to ultra-high pres-
sure systems. Ultra II™ columns are available in traditional phases (C18,
Aqueous C18, Silica) and unique chemistries which provide alternate selec-
tivity (Biphenyl,Aromax, PFPPropyl).
SulfaDrugsonUltra II™Biphenyl
Peak List
Ret. Time (min.)
1. methylparaben (methyl 4-hydroxybenzoate)
0.310
2. ethylparaben (ethyl 4-hydroxybenzoate)
0.397
3. propylparaben (propyl 4-hydroxybenzoate)
0.563
4. butylparaben (butyl 4-hydroxybenzoate)
0.875
Sample:
Inj.:
1µL
Conc.:
50µg/mL each component
Sample diluent: methanol
Column:
Ultra II™ C18
Cat.#:
9604853
Dimensions:
50mm x 3.0mm
Particle size:
2.2µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu ProminenceUFLC
XR
Mobile phase:
0.1% formic acid in
water:methanol (35:65)
Flow:
1.5mL/min.
Temp.:
30°C
Pressure:
630bar
Det.:
UV@ 254nm
LC_PH0486
Sample:
Inj.:
3µL
Conc.:
25µg/mL each component
Sample diluent: methanol
Column:
Ultra II™Biphenyl
Cat.#:
9609853
Dimensions:
50mm x 3.0mm
Particle size:
2.2µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu ProminenceUFLC
XR
Mobile phase:
A. 0.1% formic acid inwater
B. methanol
Time (min.)
%B
0
25
3.5
65
Flow:
1.0mL/min.
Temp.:
30°C
Pressure:
540bar
Det.:
UV@ 254nm
Peak List
Ret. Time (min.)
1. sulfadiazine
1.496
2 sulfathiazole
1.663
3. sulfamerazine
1.997
4. sulfamethoxazole
2.381
5. sulfamethazine
2.523
6. sulfachlorpyridizine
2.651
7. sulfamethoxine
3.228
LC_PH0489
Ultra II
™
LCColumns
NEW!
2.2μm
2.2μm
