6
•
800-356-1688
CannabinoidsonUltra II™Biphenyl
AlcoholMetabolitesonUltra II™Biphenyl
min.
0.00
0.25
0.50
0.75
1.00
1.25
1.50
1.75
2.00
2.25
2.50
2.75
3.00
Peak List
m/z
1. matrix
125
2. ethyl glucuronide
221
3. ethyl sulfate
125
Sample:
Inj.:
5µL
Conc.:
5µg/mL each component
Sample diluent: urine diluted 1:10withmobile phase
Column:
Ultra II™Biphenyl
Cat.#:
9609552
Dimensions:
50mm x 2.1mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu ProminenceUFLC
XR
Mobile phase:
A: 5mM dihexylammonium acetate inwater
B: 5mM dihexylammonium acetate inmethanol
Time (min.)
%B
0.0
40
2.5
40
2.6
100
5.4
100
5.5
40
7.5
40
Flow:
0.3mL/min.
Temp.:
40°C
Pressure:
40bar
Det.:
Shimadzu LCMS-2010EV
Ion source:
ESI-
Mode:
SIM
Source temp.:
200°C
Source voltage: -4,000V
1
2
3
LC_CF0500
LC_CF0499
Peak List:
1. cannabidiol
2. THC-COOH
3. cannabinol
4.
Δ
9
-THC-d3
5.
Δ
9
-THC
MassSpectrometerExperiments:
Analyte
Q1
Q3
cannabidiol 1
315.2
123.1
cannabidiol 2
315.2
193.2
THC-COOH 1
345.2
299.3
THC-COOH 2
345.2
193.2
cannabinol 1
311.2
223.2
cannabinol 2
311.2
178.2
Δ
9
-THC-d
3
1
318.2
196.2
Δ
9
-THC 1
315.2
193.2
Δ
9
-THC 2
315.2
123.1
Sample:
Inj.:
20µL
Conc.:
300ng/mL urine samplewith 50ng/mL internal standard
Column:
Ultra II™Biphenyl
Cat.#:
9609853
Dimensions:
50mm x 3.0mm
Particle size:
2.2µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu ProminenceUFLC
XR
Mobile phase:
A: 0.1% formic acid inwater
B: 0.1% formic acid inmethanol
Time (min.)
%B
0.0
50
0.5
50
3.5
100
4.5
100
4.6
50
6.0
50
Flow:
0.7mL/min.
Temp.:
35°C
Pressure:
380bar
Det.:
Applied Biosystems 3200QTRAP
™
LC/MS/MS system
Ion source:
TurboIonSpray
®
, ESI+
Mode:
MRM
Source temp.:
550°C
Source voltage: 5,000V
Dwell:
50ms
1
2
3
4
5
2.2μm
5μm
