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Lit. Cat.#580145
© 2007 Restek Corporation.
RestekpHidelity™C18pHStableHPLCColumns
RestekTrademarks:
pHidelity,Trident, Restek logo.
We are pleased to introduce pHidelity™ pH-stableHPLC columns, designed for analyses that require, or benefit from, extreme pH conditions.
pHidelity™columns incorporateaproprietarybarrier layer thatprotects thebase silicaparticle,anda secondary layer thatprovides the functional
stationaryphase ligand. pHidelity™ columns canbeused routinelyup topH12– a significant improvement over the typical pH2.5 to7.5 range
for silica-basedmaterials. pHidelity™ columns give youmore control over analyte retention and resolution;mobilephasepH canbe increased to
enhance retentionof basic analytes –without sacrificing column lifetime.
pHidelity™C18Columns
PhysicalCharacteristics:
3µmColumn, 4.6mm
cat.#
price
30mm
9579335
$440
50mm
9579355
$440
100mm
9579315
$455
150mm
9579365
$470
30mm (withTrident
™
Inlet Fitting)
9579335-700
$455
50mm (withTrident
™
Inlet Fitting)
9579355-700
$455
100mm (withTrident
™
Inlet Fitting)
9579315-700
$470
150mm (withTrident
™
Inlet Fitting)
9579365-700
$485
particle size: 3µm
pore size: 200Å
pH range: 1 to12
temperature limit: 80°C
pHidelity
™
C18Guard Cartridges
qty.
cat.#
price
10 x 4.0mm
3-pk.
957930210
$131
20 x 4.0mm
2-pk.
957930220
$131
UsingahighpHmobilephase isaneasyway to improvepeak retention, resolution, and symmetry ina testmixof
compoundsvarying inpKavalues.
Sample:
Inj.:
10µL
Conc.:
100µg/mL each component
Sample diluent: acetonitrile
Column:
C18
Dimensions:
150 x 4.6mm
Particle size:
3µm
Pore size:
200Å
Conditions:
Mobile phase:
20mM ammonium acetate
(pH 4):acetonitrile 50:50
Flow:
0.75mL/min.
Temp.:
ambient
Det.:
UV@ 254 nm
1. lidocaine
2/3. verapamil/diphenhydramine
4. nortriptyline
LC_PH0363
LC_PH0392
A)Poor separationofbasesunder typical conditionsona
conventional C18columnatpH4
B)Complete separationofbasesonapHidelity™columnatpH11
Column:
pHidelity
™
C18 (cat.# 9579365)
Dimensions:
150 x 4.6mm
Particle size:
3µm
Pore size:
200Å
Conditions:
Mobile phase:
10mM ammonium bicarbonate
(pH 11): acetonitrile:
tetrahydrofuran, 35:55:10 (v/v/v)
Flow:
1.5mL/min.
Temp.:
ambient
Det.:
UV@ 254 nm
1. lidocaine
2. verapamil
3. diphenhydramine
4. nortriptyline
Hours exposed to 50mM triethylamine (TEA), pH 10, 60°C
0.0000
0.1000
0.2000
0.3000
1.0000
1.1000
1.2000
1.3000
Conventional C18
pHidelity C18
90
80
70
60
50
40
30
20
10
0
Efficiency (Normalized)
pHidelity™C18columns - exceptional performanceunder
acceleratedhighpH stability testingconditions.
