688
GCCHROMATOGRAMS |
PHARMACEUTICAL
Steroids
Steroids:SexHormones
Rxi®-1ms
GC_PH00872
Column:
Rxi
®
-1ms, 30m, 0.25mm ID, 0.25µm (cat.# 13323)
Sample:
100µg/mL each hormone inmethanol or ethanol; compounds derivatized
using 2%methoxylamineHCl (CH
3
ONH
2
) in pyridine,
thenN-trimethylsilylimidazole (TMSI), then analyzed
Inj.:
1.0µL splitless (hold 0.5min.), 3.5mm single gooseneck inlet liner
(cat.# 20961)
Inj. temp.:
250°C
Carrier gas:
helium, constant flow
Flow rate:
1mL/min.
Oven temp.:
100°C to 320°C@ 10°C/min. (hold 10min.)
Det:
MS: Shimadzu 17AwithQP5000
Transfer line
temp.:
280°C
Scan range:
40-700amu
Ionization:
EI
Mode:
scan
1. androsterone
2. dehydroepiandrosterone (DHEA)
3. 17-
α
-estradiol
4. estrone
5. 17-
β
-estradiol
6. testosterone
7. derivatization by-product
Lowbleedat300+°C!
Excellent
resolutionof
closelyeluting
hormones.
Rxi®Technology!
Exceptionally inert,
ultra low- bleed
capillary columns.
RestekCustomer Service
In theU.S.
Call: 800-356-1688 (ext. 3) or 814-353-1300
(ext. 3)
Monday–Friday8:00a.m.–6:00p.m. ET
Fax: 814-353-1309—24-hoursaday
Online:www.restek.com—24-hoursaday
Outside theU.S.
Contact your Restek representative:
Refer toour listonpages4-5or visitour
websiteatwww.restek.com
StuKline, Customer Service
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
