679
GCCHROMATOGRAMS |
FORENSIC/PHARMACEUTICAL
Barbiturates, Acidic/Neutral Drugs
Barbiturates (Acidic/NeutralDrugs) (Underivatized )
Rtx®-35
Column:
Rtx
®
-35, 30m, 0.32mm ID, 0.50µm (cat.# 10439)
Sample:
1.0µL split injection of barbiturates
Oven temp.:
210°C (hold 2min.) to 300°C@ 7°C/min. (hold 2min.)
Inj./det. temp.:
300°C
Carrier gas:
helium
Linear velocity:
35cm/sec. set@ 210°C
FID sensitivity:
5.12 x 10
-10
AFS
Split vent:
30:1
1. barbital
2. aprobarbital
3. butalbital
4. amobarbital
5. talbutal
6. pentobarbital
7. methohexital
8. secobarbital
9. thiopental
10. hexobarbital
11. mephobarbital
12. phenobarbital
GC_PH00267
12
11
10
9
8
7
6
4
5
1
2
3
min.
2
4
6
8
10
Separationoptimized
usingPro
ez
GC
software!
Seepage580 fordetails.
Acidic/NeutralDrugs
Rxi®-5ms
Column:
Rxi
®
-5ms, 30m, 0.25mm ID, 0.25µm (cat.# 13423)
Sample:
1,000µg/mL each inmethanol
Inj.:
1.0µL split (50:1), 20ng each compound on column;
Siltek
®
treated 4mm gooseneck splitless inlet liner
(cat.# 20799-214.5)
Inj. temp.:
250°C
Carrier gas:
helium
Linear velocity: 30cm/sec., constant pressure
Oven temp.:
100°C to 220°C@ 15°C/min., to 330°C@
10°C/min. (hold 5min.)
Det.:
Agilent 5973MSD
Transfer line
temp.:
300°C
Scan range:
35-550
Tune:
PFTBA
Ionization:
EI
1. methprylon
2. butalbital
3. amobarbital
4. meprobamate
5. glutethimide
6. phenobarbital
7. methaqualone
8. primidone
GC_PH00815
Rxi®Technology!
Exceptionally inert,
ultra low- bleed
capillary columns.
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
