674
GCCHROMATOGRAMS |
CLINICAL, FORENSIC&TOXICOLOGY
Opiates, Cannabinoids
DerivatizedCannabinoids (5μg/mL)
Rxi®-5SilMS
Column
Rxi
®
-5Sil MS, 30m, 0.25mm ID, 0.25µm (cat.# 13623)
Sample
Diluent:
Ethyl acetate
Conc.:
5µg/mLTMS derivatives
Injection
Inj. Vol.:
1µL splitless (hold 1min.)
Liner:
3.5mmGooseneck Splitlessw/Wool (cat.# 22286-200.1)
Inj.Temp.:
250 °C
Purge Flow:
21.4mL/min.
Oven
OvenTemp:
150 °C to 330 °C at 15 °C/min. (hold 3min.)
Carrier Gas
He, constant linear velocity
Linear Velocity: 40 cm/sec., 13.8 psi, 95.1kPa@ 150 °C
Detector
MS
Mode:
Scan
Transfer Line
Temp.:
280 °C
AnalyzerType: Quadrupole
SourceTemp.:
200 °C
Electron Energy: 70 eV
Solvent Delay
Time:
4min.
TuneType:
PFTBA
IonizationMode: EI
Scan Range:
200-500 amu
Scan Rate:
5 scans/sec.
Instrument
Shimadzu 2010GC&QP2010+MS
GC_CF1145
Excellentpeak shape
with short run time.
NEW!
Peaks
1. Cannabidiol
2. delta 9THC
3. Cannabinol
4. THC-COOH;THCA
GC_CF1150
ExtractedOpiates fromBlood
Rxi®-5SilMS
Peaks
1. Hydrocodone
2. Codeine
3. Oxycodone
4. Hydromorphone
5. 6-Monoacetylmorphine
6. Morphine
* Matrix
Column
Rxi
®
-5Sil MS, 30m, 0.25mm ID, 0.25µm (cat.# 13623)
Sample
Diluent:
Ethyl acetate
Conc.:
100 ng/mL propionic anhydride derivatives
Injection
Inj. Vol.:
1µL splitless (hold 1min.)
Liner:
Gooseneck Splitless (4mm) w/Wool (cat.# 22405)
Inj.Temp.:
250 °C
Purge Flow:
100mL/min.
Oven
OvenTemp:
65 °C (hold 1min.) to 315 °C at 15 °C/min.
Carrier Gas
He, constant flow
FlowRate:
1mL/min.
Linear Velocity:
35 cm/sec.@ 65 °C
Detector
MS
Mode:
SIM
Transfer Line
Temp.:
250 °C
AnalyzerType:
Quadrupole
Solvent Delay
Time:
7min.
TuneType:
PFTBA
IonizationMode: EI
Notes
Opiateswere spiked into a blood sample and extracted
by SPE, then derivatizedwith propionic anhydride.
Acknowledgement Data courtesy of Miami Dade County
Medical Examiner Department
NEW!
Rxi®Technology!
Exceptionally inert,
ultra low- bleed
capillary columns.
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
