669
GCCHROMATOGRAMS |
CLINICAL, FORENSIC&TOXICOLOGY
GHB/GBL
γ
-butyrolactone&1,4-butanediol
Rtx®-BAC1&Rtx®-BAC2
Columns:
Rtx
®
-BAC1, 30m, 0.32mm ID, 1.8µm (cat.# 18003)
Rtx
®
-BAC2, 30m, 0.32mm ID, 1.2µm (cat.# 18002)
Sample:
100µg/mL each inmethanol
Inj.:
1.0µL split (split ratio 1:10), 4mm single gooseneck
inlet liner withwool (cat.# 22405)
Inj. temp.:
240°C
Carrier gas:
helium, constant flow
Flow rate:
3.0mL/min.
Oven temp.:
50°C to 240°C@ 10°C/min. (hold 2min.)
Det.:
FID@ 240°C
1. 2,3-butanediol
2. 1,2-butanediol
3. 1,3-butanediol
4. 1,4-butanediol
5.
γ
-butyrolactone
GC_PH00808
GC_PH00809
Rtx®-BAC1
Rtx®-BAC2
γ
-hydroxybutyrate (GHB)and
γ
-butyrolactone (GBL)
Rtx®-BAC1andRtx®-BAC2 (dual columnanalysis)
GC_PH00870
GC_PH00871
Columns:
Rtx
®
-BAC1, 30m, 0.32mm ID, 1.8µm (cat.# 18003) and
Rtx
®
-BAC2, 30m, 0.32mm ID, 1.2µm (cat.# 18002),
connected via universal “Y” Press-Tight
®
connector (cat.# 20405)
Sample:
GHB, GBL,
α
-methylene-
γ
-butyrolactone (AMGBL), 10µg/mL each inwater
Inj.:
1.0mL headspace, split (split ratio 1:10), 1mm split inlet liner (cat.# 20972)
Inj. temp.:
200°C
Carrier gas:
helium, constant pressure
Linear velocity:
44cm/sec.@ 50°C
Oven temp.:
50°C (3min.) to 150°C@ 20°C/min. (hold 7min.)
Det:
FID@ 240°C
Headspace autosampler: Teledyne/Tekmar HT3
Sample/platen temp.:
100°C
Sample equilibration:
15min.
Mixing time:
5min.
Vial pressure:
10psig
Vial pressurization
time:
2min.
Loop fill time:
2min.
Transfer line temp.:
120°C
1.
γ
-hydroxybutyrate (GHB)/
γ
-butyrolactone (GBL)
2.
α
-methylene-
γ
-butyrolactone (AMGBL)
Rtx®-BAC1
Rtx®-BAC2
Sharppeaksandbaseline
resolution in less than5minutes—
12 samplesperhour!
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
