CLINICAL/FORENSICS
18
Analgesic Formulations
HPLC
HPLC
AcetaminophenandNarcoticAnalgesics
Allure™Basix
1
Peak List:
1. acetaminophen
2. codeine
3. oxycodone
4. hydroxycodone
Sample:
Inj.:
5µL
Conc.:
100µg/mL
Solvent:
mobile phase
Column:
Allure™Basix
Cat.#:
9161565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
20mM ammonium
acetate, pH 4.5:
acetonitrile (85:15, v/v)
Flow rate:
1.0mL/min.
Temp.:
30°C
Det.:
UV@ 280nm
2
3
0 1
2
3
4
5
6
7
8
9 min.
LC_0082
4
Peak List:
Conc. (µg/mL)
Ret. Time (min.)
Tailing Resolution
U. unknown
unknown
3.1
NA
NA
1.morphine sulfate
204
3.3
1.0
1.8
2.acetaminophen
92
5.0
1.1
14.1
3. codeine phosphate
216
5.5
1.4
2.6
4.oxycodoneHCl
206
7.5
1.4
8.5
5.hydrocodone bitartrate 218
8.9
1.4
5.0
LC_0218
AcetaminophenandNarcoticAnalgesics
UltraC18
Column:
Ultra C18
Catalog#:
9174575
Dimensions:
250 x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase: A: 10mm
potassium phosphate,
pH 2.8
B: acetonitrile:
methanol (90:10 v/v)
(85A:15B, v/v)
Flow:
1.0mL/min.
Temp.:
27°C
Det.:
UV@ 235nm
Sample:
Inj.:
4.0µL
Sample:
rawmaterial mix
Solvent:
mobile phase
Analgesics
Popular components for multi-active analgesic
formulations includeaspirin, salicylicacid, salicy-
lamide, acetaminophen, ibuprofen, naproxen,
guaifenesin, codeine, oxycodone, hydrocodone,
and caffeine. Many of these compounds can be
analyzed simultaneouslyusinghighperformance
liquid chromatography (HPLC), thereby improv-
ing laboratory efficiency and productivity.With
the selection of the proper LC phase, separation
becomes a simple andmanageable task that does
not rely upon extensive preparation procedures
or use of ion pairing agents, which often are
described inpharmaceutical compenda.
Ultra C18, Ultra Phenyl, and Allure™ Basix
HPLC column phases separatemixtures of these
pharmaceuticals inaproductiveandcost effective
manner.The selective chemistries of thesephases
provide powerful separationmechanisms.
free
literature
ImprovedHPLCAnalysis of Analgesics
lit. cat.# 59511A
GenuineRestek Replacement Parts for HPLC Systems
lit. cat.# 59012A
HPLC Tech TipsWall Chart
lit. cat.# 59894A
Call Restek at 800-356-1688 or 814-353-1300, ext. 5, or contact
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