CLINICAL/FORENSICS
22
Antiepileptics
GC
GC
Antiepileptics
Laboratories thatperform therapeuticdrugmon-
itoring tests analyze antieplileptic (anti-convul-
sant) drugs using either GC or HPLC because
immunoassays donot exhibit linearity and show
cross reactivities in the toxic range. Common
antiepileptics are resolved using an Rtx
®
-20 or
Rtx
®
-1701GC column.
Antiepileptics (Underivatized)
Rtx®-20
1. valproic acid
2. ethosuximide
3. methsuximide
4. phensuximide
5. desmethyl methsuximide
6. mephenytoin
7. ethotoin
8. PEMA
9. phenobarbital
10. primidone
11. carbamazepine
12. diphenylhydantoin
15m, 0.32mm ID, 0.50µmRtx
®
-20 (cat.# 10336)
1.0µL split injection of antiepileptic drugs
Conc.:
1000ng/µL
Oven temp.:
150°C to 280°C@
7°C/min. (hold 5min.)
Inj. / det. temp.:
250°C / 270°C
Det.:
FID
Carrier gas:
helium
Linear velocity:
30cm/sec. set@ 150°C
FID sensitivity:
1.28 x 10
-10
AFS
Split ratio:
30:1
1
min.
5
10
15
20
2
3 4
5
6
7 8 9
10
11
12
GC_PH00265
Antiepileptics (Underivatized)
Rtx®-1701
1. valproic acid
2. ethosuximide
3. methsuximide
4. phensuximide
5. desmethyl methsuximide
6. mephenytoin
7. ethotoin
8. PEMA
9. phenobarbital
10. primidone
11. carbamazepine
12. diphenylhydantoin
15m, 0.32mm ID, 0.50µmRtx
®
-1701 (cat.# 12036)
1.0µL split injection of antiepileptic drugs
Conc.:
1000ng/µL
Oven temp.:
150°C to 280°C@
7°C/min. (hold 5min.)
Inj. / det. temp.:
250°C / 270°C
Det.:
FID
Carrier gas:
helium
Linear velocity:
30cm/sec. set@ 150°C
FID sensitivity:
1.28 x 10
-10
AFS
Split ratio:
30:1
GC_PH00266
1
min. 5
10
15
20
2
3
4
5
6
7
8
9
10
11
12
