CLINICAL/FORENSICS
24
Cardiac Medications; CNS Depressants (Sedatives)
HPLC
Sedatives
Sedatives are CNS (central nervous system)
depressants. They have been identified as con-
tributors in impaired driving cases, and have
been used in greyhound racing and horseracing
to decrease an animal's speed. CNS depressants
canbeanalyzedbyHPLConanAllure
™
Basixcol-
umn.
Peak List:
1. toluene (marker)
2. lorazepam
3. alprazolam
Sample:
Inj.:
10µL
Conc.:
500µg/mL
Solvent:
water:acetonitrile
(7:3, v/v)
Column:
Allure™Basix
Cat.#:
9161565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
10mM ammonium
formate, pH 3.0:acetonitrile
(70:30, v/v)
Flow rate:
2.0mL/min.
Temp.:
25°C
Det.:
UV@ 230nm
1
2
3
AlprazolamandLorazepam (Sedatives)
Allure™Basix
LC_0078
0
1
2
3
4
5 min.
HPLC
Metoprolol (Antiarrhythmic)
Allure™Basix
Peak List:
1. unknown
2. metoprolol
Sample:
Inj.:
2.5µL
Conc.:
1.5mg/mL
Solvent:
methanol:water
(30:70)
Column:
Allure™Basix
Cat.#:
9161565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
10mM ammonium
formate, pH 2.5:
acetonitrile (10:90, v/v)
Flow rate:
1.2mL/min.
Temp.:
25°C
Det.:
UV@ 225nm
LC_0090
CardiacMedications
Several classesofmedicationsareused todecrease
highbloodpressure, control arrhythmias (abnor-
mal heart rhythms), and treat congestive heart
failure. These medications include beta antago-
nists,ACE inhibitors,diuretics,andcalciumchan-
nel blockers.HPLC is the preferred technique for
analyzing many of these compounds. Selecting
the appropriate analytical column is critical,
becausemany of the basic compounds tail badly
onpoorlydeactivatedHPLCphases.Restek’s fully
end-capped Allure
™
Basix, Allure
™
PFP Propyl,
Ultra PFP, and Ultra Cyano phases can use the
basicnatureof these compounds to achieve sepa-
rationwithout peak tailing.
free
literature
HPLCAnalysis of Basic Pharmaceutical Compounds
on anUltra Cyano Phase
lit. cat.# 59545
Call Restek at 800-356-1688 or 814-353-1300, ext. 5, or contact
your Restek representative, to request your free copy!
free
literature
Analyzing CardiacMedications by HPLC
lit. cat.# 59151
Call Restek at 800-356-1688 or 814-353-1300, ext. 5, or contact
your Restek representative, to request your free copy!
