CLINICAL/FORENSICS
23
Cardiac Medications
HPLC
HPLC
Atenolol (Antiarrhythmic)
Allure™Basix
1
Peak List:
1. uracil (marker)
2. atenolol
Sample:
Inj.:
1µL
Conc.:
1mg/mL
Solvent:
water:methanol
(7:3, v/v)
Column:
Allure™Basix
Cat.#:
9161565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
acetonitrile:20mM ammonium
acetate, pH 4.5 (80:20, v/v)
Flow rate:
1.2mL/min.
Temp.:
25°C
Det.:
UV@ 225nm
2
0
2
4
6
8
10
12
min.
LC_0072
TriamtereneandHydrochlorothiazide
(Antiarrhythmics)
Allure™Basix
Peak List:
1. hydrochlorothiazide
2. triamterene
Sample:
Inj.:
1µL
Conc.:
500µg/mL
Solvent:
methanol:water:
tetrahydrofuran
(30:35:35)
Column:
Allure™Basix
Cat.#:
9161565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
20mM ammonium
acetate, pH 4.5:acetonitrile
(65:35,v/v)
Flow rate:
1.2mL/min.
Temp.:
25°C
Det.:
UV@ 225nm
LC_0086
HPLC
CalciumChannelBlockers (Antiarrhythmics)
UltraCyano
1
LC_0062
0
1
2
3
4
5
6
7
8
min.
2
3
4
5
Peak List:
1. diltiazem
2. nifedipine impurity
3. verapamil
4. nifedipine
5. nicardipine
Sample:
Inj.:
5µL
Conc.:
100mg/mL
Solvent:
acetonitrile:water (1:1)
Column:
Ultra Cyano
Cat.#:
9106565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
20mM potassium
phosphatemonobasic,
pH 3.0:acetonitrile
(70:30, v/v)
Flow rate:
1.2mL/min.
Temp.:
30µC (±1°C)
Det.:
235nm
LC_0250
Digitalis
Extracts/Derivatives
UltraPFPPropylCartridgeColumn
(FastLC)
Peak List:
Conc.
Ret. Time (min.)
1. digoxigenin 100µg/mL
0.40
2. gitoxigenin 100µg/mL
0.80
3. digoxin
100µg/mL
1.10
4. gitoxin
~10µg/mL 2.20
5. digitoxin 100µg/mL
2.60
Sample:
Inj.:
10µL
Solvent:
water:acetonitrile (80:20 v/v)
Column:
Ultra PFPPropyl (Custom)
Dimensions:
30 x 4.0mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Mobile phase:
A: water
B: acetonitrile
Time (min.) %B
0.0
20
1.5
20
1.51
35
3.0
35
3.1
20
Flow:
2.0mL/min.
Temp.:
27°C
Det.:
UV@ 230nm
