CLINICAL/FORENSICS
27
Steroids
HPLC
HPLC
HPLC
Peak List:
1. uracil
2. triamcinolone
3. hydrocortisone
4. dexamethasone
5. corticosterone
6. deoxycorticosterone
Sample:
Inj.:
5µL
Conc.:
100µg/mL
Solvent:
mobile phase
Corticosteroids
UltraC18
0
1
2
3
4
5
6
min.
LC_0108
3
2
1
4
5
6
Column:
Ultra C18
Cat.#:
9174565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
water:methanol
(30:70, v/v)
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV@ 220nm
0
1
2
3
4
5
6
7
8 min.
1
2
3
4
Corticosteroids
Pinnacle™ IIPhenyl
Peak List:
1. hydrocortisone
2. cortisone
3. corticosterone
4. cortisone acetate
Sample:
Inj.:
5µL
Conc.:
200µg/mL each
Solvent:
methanol
Column:
Pinnacle
™
II Phenyl
Cat.#:
9215565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase:
water:methanol (60:40 v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
Corticosteroids
Allure™Biphenyl
LC_PH0330
Ret. Time
Peak List
(min.)
1. hydrocortisone
4.19
2. prednisone
4.79
3. cortisone
5.08
4. dexamethasone
6.37
5. corticosterone
9.01
6. cortisone acetate
15.75
7. desoxycorticosterone
25.94
Sample:
Inj.:
5µL
Conc.:
100µg/mL each component
Sample diluent:
methanol
Column:
Allure™Biphenyl
Cat.#:
9166565
Dimensions:
150 x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
water:acetonitrile, 60:40
Flow:
1mL/min.
Temp.:
ambient
Det.:
UV@ 254 nm
LC_0182
