CLINICAL/FORENSICS
10
Blood Alcohol
GC
GC
BloodAlcohol
Resolution and analysis time are two critical factors to considerwhendeveloping an assay for ethanol andother volatiles in
biological samples.Wehave developed two stationaryphases specifically for blood alcohol analysis; eachhas the ability tobase-
line resolve all of the lowmolecularweight alcohols and theirmetabolites. Elutionorder of this analyte set differs on the two
stationaryphases, enabling screening and confirmationof volatile compounds tobe performedwithone injection.These
columns alsowere designed formaximum sample throughput,with total analysis timeunder 3.5minutes for an isothermal
run. In addition toblood alcohol analysis, these columns canbeused to testwhole blood, serum, or urine for volatile com-
pounds, using temperature programming.
BloodAlcohol
Rtx®-BAC1&Rtx®-BAC2 (0.32mm IDColumns)
30m, 0.32mm ID, 1.8µm Rtx
®
-BAC1 (cat.# 18003)
30m, 0.32mm ID, 1.2µm Rtx
®
-BAC2 (cat.# 18002)
Dual-column analysis using a two-hole ferrule.
1.0mL headspace sample of a blood alcohol mix inwater
Oven temp.:
40°C
Inj. temp.:
200°C
Carrier gas:
helium
Sample equilibration:
70°C, 15min.
Vial pressure:
30psi
Vial pressurization time:
0.15min.
Vial sampling time:
0.01min.
Transfer line:
0.32mm IDHydroguard
™
fused silica tubing
Transfer line temp.:
200°C
Injection port sleeve:
2mm ID
Split flow:
20mL/min.
Conc.
w/v
1. methanol
0.1%
2. acetaldehyde
0.2%
3. ethanol
0.2%
4. isopropanol
0.1%
5. acetone
0.01%
6.
n
-propanol
0.1%
6
5
4
3
2
1
6
5
4
3
2
1
GC_PH00241
min.
1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0
BloodAlcohol
Rtx®-BAC1&Rtx®-BAC2 (0.53mmIDcolumns)
6
5
1
2
3
4
1. methanol
2. acetaldehyde
3. ethanol
4. isopropanol
5. acetone
6.
n
-propanol
min.
1
2
3
1
2
3
4
5
6
30m, 0.53mm ID, 3.0µmRtx
®
-BAC1 (cat.# 18001)
30m, 0.53mm ID, 2.0µmRtx
®
-BAC2 (cat.# 18000)
1.0mL headspace sample of a blood alcohol mix inwater
Sample conc.:
0.1% per compound
Oven temp.:
40°C
Inj. & det. temp.:
200°C
Carrier gas:
helium
Linear velocity:
80cm/sec. set@ 40°C
FID sensitivity:
1.28 x 10
-10
AFS
GC_PH00239
min.
1
2
3
min.
1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0
Rtx®-BAC1
Rtx®-BAC2
Rtx®-BAC1
Rtx®-BAC2
