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Figure 2—Rapid, efficient analysis of FAMEs derived from amarine oil capsule, using a FAMEWAX
®
column.
GC_FF00568
1. C14:0
2. C15:0
3. C16:0
4. C16:1
5. C16:2
6. C17:0
7. C17:1
8. C16:4
9. C18:0
10. C18:1 (oleate)
11. C18:1 (vaccenate)
12. C18:2n6cis
13. C18:3n3
14. C18:4n6
15. C18:4n3
16. C20:0
17. C20:1n7
18. C20:1n9
19. C20:4n6
20. C20:4n3
21. C20:5n3
22. C22:1n7
23. C21:5n3
24. C23:0 (IS)
25. C22:5n6
26. C22:5n3
27. C22:6n3
28. C24:1
FAMEWAX
™
30m, 0.32mm ID, 0.25µm (cat.# 12498)
Sample:
12mg/mL total FAMEs
Inj.:
0.5µL, split (150:1), 3mm ID split liner for
Trace SeriesGCs, packedwith glasswool
(cat.# 20936-202.1)
Inj. temp.:
250°C
Carrier gas:
hydrogen, constant flow
Linear velocity: 62cm/sec.
Oven temp.:
195°C to 240°C@ 5°C/min. (hold 1min.)
Det.:
FID, 250°C
Figure 3—Effective analysis of a 37 FAMEmix on anRt-2560 capillary column.
GC_FF00649
Rt-2560, 100m, 0.25mm ID, 0.2µm (cat.# 13199)
Sample:
30mg/mL total FAMEs inmethylene chloride
Inj.:
2.0µL split (split ratio 200:1), 4mm inlet liner (cat.# 20814)
Inj. temp.:
225°C
Carrier gas:
hydrogen, constant flow
Flow rate:
1.2mL/min.
Oven temp.:
100°C (4min. hold)
to 240°C@ 3°C/min. (10min. hold)
Det.:
FID@ 250°C
Compound
% inMix
1. C4:0 methyl butyrate
4.0
2. C6:0 methyl hexanoate
4.0
3. C8:0 methyl octanoate
4.0
4. C10:0 methyl decanoate
4.0
5. C11:0 methyl undecanoate
2.0
6. C12:0 methyl laurate
4.0
7. C13:0 methyl tridecanoate
2.0
8. C14:0 methyl myristate
4.0
9. C14:1 methyl myristoleate (
cis
-9)
2.0
10. C15:0 methyl pentadecanoate
2.0
11. C15:1 methyl pentadecenoate (
cis
-10)
2.0
12. C16:0 methyl palmitate
6.0
13. C16:1 methyl palmitoleate (
cis
-9)
2.0
14. C17:0 methyl heptadecanoate
2.0
15. C17:1 methyl heptadecenoate (
cis
-10)
2.0
16. C18:0 methyl stearate
4.0
17. C18:1 methyl elaidate (
trans
-9)
2.0
18. C18:1 methyl oleate (
cis
-9)
4.0
19. C18:2 methyl linoleaidate (
trans
-9,12)
2.0
20. C18:2 methyl linoleate (
cis
-9,12)
2.0
21. C18:3 methyl
γ
-linolenate (
cis
-6,9,12)
2.0
22. C20:0 methyl arachidate
4.0
23. C20:1 methyl eicosenoate (
cis
-11)
2.0
24. C18:3 methyl linolenate (
cis
-9,12,15)
2.0
25. C21:0 methyl heneicosanoate
2.0
26. C20:2 methyl eicosadienoate (
cis
-11,14)
2.0
27. C20:3 methyl eicosatrienoate (
cis
-11,14,17)
2.0
28. C22:0 methyl behenate
4.0
29. C22:1 methyl erucate (
cis
-13)
2.0
30. C20:3 methyl eicosatrienoate (
cis
-11,14,17)
2.0
31. C20:4 methyl arachidonate (
cis
-5,8,11,14)
2.0
32. C23:0 methyl tricosanoate
2.0
33. C22:2 methyl docosadienoate (
cis
-13,16)
2.0
34. C20:5 methyl eicosapentaenoate (
cis
-5,8,11,14,17)
2.0
35. C24:0 methyl lignocerate
4.0
36. C24:1 methyl nervonate (
cis
-15)
2.0
37. C22:6 methyl docosahexaenoate (
cis
-4,7,10,13,16,19)
2.0
