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HPLCanalyses described in this 8-page publication include
carbamates, carbonyls, explosives, paraquat/diquat, phenoxyacid
herbicides, and polyaromatic hydrocarbons.
Lit. cat.# 59741A
PolyaromaticHydrocarbons (PAHs)byHPLC
Pinnacle™ IIPAHColumns
PhysicalCharacteristics:
ordering note
For guard cartridges for these columns, visit ourwebsite at
.
particle size: 5µm,
spherical
pore size: 110Å
endcap: fully endcapped
pH range: 2.5 to10
temperature limit: 80°C
acenaphthene
acenaphthylene
anthracene
benzo(a)anthracene
benzo(a)pyrene
benzo(b)fluoranthene
benzo(ghi)perylene
benzo(k)fluoranthene
chrysene
dibenzo(a,h)anthracene
fluoranthene
fluorene
indeno(1,2,3-cd)pyrene
1-methylnaphthalene
2-methylnaphthalene
naphthalene
phenanthrene
pyrene
EPAMethod8310PAHMixture
(18 components)
500µg/mL each in acetonitrile, 1mL/ampul
cat.# 31841 (ea.)
$62
acenaphthene 100µg/mL
acenaphthylene
100
anthracene
100
benzo(a)anthracene
10
benzo(a)pyrene
10
benzo(b)fluoranthene 10
benzo(ghi)perylene
10
benzo(k)fluoranthene
5
chrysene
10
dibenzo(a,h)anthracene 10
fluoranthene
10
fluorene
100
indeno(1,2,3-cd)pyrene 10
1-methylnaphthalene 100
2-methylnaphthalene 100
naphthalene
100
phenanthrene
100
pyrene
10
EPAMethod8310QualityControlCheck
(18 components)
In acetonitrile, 1mL/ampul
cat.# 31843 (ea.)
$46
decafluorobiphenyl
EPAMethod8310SurrogateStandard
1,000µg/mL in acetonitrile, 1mL/ampul
cat.# 31842 (ea.)
$23
MostHPLCPAHmethods recommendusingaC18columnwith fluorescence
and/or UV/VIS detection. Our Pinnacle™ II PAH columns have a highly
reproduciblemodified alkyl phase onRestekmanufactured silica, specifically
developed for this application. Figure 1 shows the analysis of 16 target PAHs
in less than18minutes,andFigure2 showsa separationof 20 targetPAHsand
related compounds, in less than6minutes, using a 5cm column.
Figure1
Baseline separationof 16PAHs in less than18minutes
onaPinnacle™ II PAHcolumn.
6
8
10
12
14
16
18
min.
1
2
3
4
6
7 8
9
11
13
12
15
16
14
10
5
Sample:
Inj.:
5µL
Solvent:
1:9methylene chloride:acetonitrile
Column:
Pinnacle
™
II PAH (Cat.# 9219563)
150mm x 3.2mm ID
Conditions:
Mobile phase: A: water, B: acetonitrile
Time (min.) %B
0
40
7
60
11
100
17.9
100
18
40
Flow:
1.2mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
LC_0303
Peak List:
Conc. (µg/mL)
1. naphthalene
100
2. acenaphthylene
100
3. acenaphthene
100
4. fluorene
100
5. phenanthrene
50
6. anthracene
100
7. fluoranthene
50
8. pyrene
50
9. benzo(a)anthracene
50
10. chrysene
50
11. benzo(b)fluoranthene 50
12. benzo(k)fluoranthene 50
13. benzo(a)pyrene
50
14. dibenzo(a,h)anthracene 50
15. benzo(ghi)perylene
50
16. indeno(1,2,3-cd)pyrene 50
Figure2
Fast, efficient separationof 20 targetPAHsand related
compoundsusinga5cmPinnacle™ II PAHcolumn.
Peak List:
1. naphthalene
2. acenaphthylene
3. 1-methylnaphthalene
4. 2-methylnaphthalene
5. acenaphthene
6. fluorene
7. phenanthrene
8. anthracene
9.
o
-terphenyl
10. fluoranthene
11. pyrene
12.
p
-terphenyl
13. benzo(a)anthracene
14. chrysene
15. benzo(b)fluoranthene
16. benzo(k)fluoranthene
17. benzo(a)pyrene
18. dibenzo(a,h)anthracene
19. benzo(ghi)perylene
20. indeno(1,2,3-cd)pyrene
Sample:
Inj.:
5µL
Conc.:
5-10ppm each
Sample diluent:
methylene chloride:acetonitrile (1:9 v/v)
Column:
Pinnacle
™
II PAH (Cat.# 9219555)
50mm x 4.6mm ID
Conditions:
Mobile phase:
A: water
B: acetonitrile
Time (min.)
%B
0
50
1.5
60
4.0
100
5.5
100
5.6
50
Flow:
3.0mL/min.
Temp.:
ambient
Det.:
UV@ 254nm
LC_0316
5µmColumn
cat.#
price
50 x 2.1mm
9219552
$364
50 x 3.2mm
9219553
$364
150 x 3.2mm
9219563
$389
50 x 4.6mm
9219555
$364
150 x 4.6mm
9219565
$389
10 x 2.1mm
921950212
$131
10 x 4.0mm
921950210
$131
20 x 2.1mm
921950222
$131
20 x 4.0mm
921950220
$131
RecommendedColumns
AnalyticalReferenceMaterials
12
